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| Year : 2008 | Volume
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| Issue : 2 | Page : 108-110 |
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| Analgesic activity of seeds of Moringa oleifera Lam. |
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Nitin G Sutar1, CG Bonde2, VV Patil2, SB Narkhede2, AP Patil2, RT Kakade2
1 SND College of Pharmacy, Babhulaon, Tal-Yeola, Dist Nashik - 423 401, India
2 Department of Pharmaceutical Chemistry, College of Pharmacy, Faizpur Tal. Yawal Dist., Jalgaon - 425 503, Maharashtra, India
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 Abstract | | |
Moringa oleifera Lam. Seed has been documented to posses antimicrobial and water purifying activities and also used in the treatment of gout, eye infections and in arthritis. The alcoholic extract of Leaves of Moringa oleifera Lam. were reported to have analgesic activity but seed still not reported. The effect of alcoholic extract and its various fractions as Petroleum ether, Ethyl acetate, Diethyl ether, n-Butanol were tested for qualitative analysis which contain glycosides, Flavonoids, tannins, amino acids (alpha-4rhamnoloxy benzyl isothiocynate). The extracts were also tested for their Analgesic activity was carried out by using Hotplate and Tail immersion method. Aspirin (25 mg/kg) was used as a standard. Keywords: Analgesic activity by hotplate, aspirin, seeds of Moringa oleifera lam., tail immersion apparatus
How to cite this article:
Sutar NG, Bonde C G, Patil V V, Narkhede S B, Patil A P, Kakade R T. Analgesic activity of seeds of Moringa oleifera Lam. Int J Green Pharm 2008;2:108-10 |
How to cite this URL:
Sutar NG, Bonde C G, Patil V V, Narkhede S B, Patil A P, Kakade R T. Analgesic activity of seeds of Moringa oleifera Lam. Int J Green Pharm [serial online] 2008 [cited 2013 May 19];2:108-10. Available from: http://www.greenpharmacy.info/text.asp?2008/2/2/108/41182 |
| Introduction | |  |
Moringa oleifera Lam. [Moringiaceae] is found in throughout India. It is known as drumstick in English, Mungna in Hindi, shevgi in Marathi. [1] Dried seeds of Moringa Oleifera are used in ophthalmic preparation, venereal affection anti-inflammatory, purgative and as tonic. [2],[3],[4] The alcoholic extract of the leaves of Moringa oleifera Lam. are reported to have analgesic activity [5] but seed still not reported. The effect of alcoholic extract and its Various fractions as Petroleum ether, Ethyl acetate, Diethyl ether, n-Butanol. The aim of the study was to screen the effect of the seeds of Moringa oleifera Lam. as analgesic.
| Materials and Methods | | |
The fresh seeds of Moringa oleifera Lam. were collected. They were shade dried and ground to obtain coarse particle size. The powdered material was extracted with 95% alcohol in a continuous hot extractor at 40°-50°C temperature. Some part of the extract was kept aside and the remaining was fractionated with Pet. ether, Ethyl acetate, Diethyl ether, and n-Butanol. What ever the fractions collected was washed with water and then air dried and kept separately with Na 2 SO 3 as dehydrating agent.
Qualitative analysis were performed for the alcoholic extract showed that the presence of glycosides, amino acids, and sterols. Pet. Ether extract showed presence of fats and oils. [6],[7],[8]
| Evaluation of Analgesic Activity | | |
Hotplate Method
In this method Wister male albino rats (180-200 g) were used for the study. The animals were segregated into seven groups of six animals each.
Group 1 - Normal saline solution,
Group 2 - Aspirin as standard (25 mg/kg),
Group 3 - Alcohol extract (30 mg/kg),
Group 4 - Pet ether fraction (100 mg/kg),
Group 5 - Ethyl acetate fraction (300 mg/kg),
Group 6 - Diethyl ether fraction (300 mg/kg),
Group 7 - n-Butanol fraction (300 mg/kg).
The dried extract and its fraction were formulated as a suspension in distilled water. Alcoholic extract and its various fractions were administered orally using intragastric tube. The pain threshold (Number of licking of paw/jumping) were measured at 20, 60, 90 min after administration of standard and test solution. [9]
Tail Immersion Method
In this method Wister male albino rats (170-210 g) were used. The lower 5 cm portion of the tail was marked and this part of tail was immersed in a cap of water having temperature 55°C. Reaction time was recorded before and after the administration of drug, extract and fractions. The animals were segregated into seven groups of six animals each.
Each group was administered with same amount of saline, standard drug (Aspirin) and extracts as given in hotplate method procedure. The pain threshold [Time required for removal of tail from hot water (55°C) was measured at 0, 1, 2, 3 and 4 h after administration of standard and test solution.
Group 1 - Normal saline solution,
Group 2 - Aspirin as standard (25 mg/kg),
Group 3 - Alcohol extract (30 mg/kg)
Group 4 - Pet ether fraction (100 mg/kg),
Group 5 - Ethyl acetate fraction (300 mg/kg),
Group 6 - Diethyl ether fraction (300 mg/kg),
Group 7 - n-Butanol fraction (300 mg/kg).
The dried alcoholic extract and its various fractions were formulated as a suspension in distilled water. Alcoholic extract and its various fractions were administered orally using intragastric tube. The rectal temperatures were measured at 30, 60, 90, 120, and 180 min after administration of standard and test solution. [10],[11]
| Results and Discussion | | |
Analgesic Activity
Amongst alcoholic extract and its various fractions of seeds of Moringa oleifera Lam., alcoholic extract showed potent analgesic activity which is comparable to that of aspirin at the dose of 25 mg/kg of body weight [Table 1],[Table 2].
From this study, it can be concluded that the seeds of Moringa oleirera Lam. possess marked analgesic activity [Figure 1] and [Figure 2] and is equipotent to standard drug (Aspirin). The present study establishes the use of Moringa oleifera Lam. seeds as regular analgesic. The plant plant has to be further explored for its phytochemical profile to identify the active constituent responsible for the above mentioned activities.
| Acknowledgements | | |
The authors wish to thanks Themis Laboratories (Thane India) for providing gift sample Aspirin by Mr. H.S. Bonde. The authors also wish to thank the management of the college for encouraging and providing research facilities.
| References | | |
| 1. | The Wealth of India. Council of Scientific and Industrial Research New Delhi vol. (VI), 1999; 426- 9.  |
| 2. | Parrotta JA. In: Healing Plants of Peninsular Indian. CSBI Publishing; 2001:528-9. |
| 3. | Prajapati ND, Purohit S.S., Sharma A.K., Kumar T. In: A Hard Book of Medicinal Plant A Complete Source Book, 1st ed. Jodhpur: Agro bios; 2003. p. 218. |
| 4. | Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants Central Drug Research institute Luck now and National institute of Science communication, Vol 1. New Delhi:280. |
| 5. | Rao CV, Ojha SK. Analgesic effect of Moringa oleifera Lam. leaf extract on rats, 2 nd World Congress on "Biotechnological Developments of Herbal Medicine". Luck now, India: NBRI; 2003. p. 42. MAPA -02-911. |
| 6. | Kokate CK. Practical Pharmacognory. 4 th ed. Delhi: Vallabh Prakashan; 1994. p. 110. |
| 7. | Sethi PD. Identification of Drugs in Pharmaceutical formulation by Thin Layer Chromatography. 2 nd ed. CBS Publisher. |
| 8. | Raphael IK. Natural products: A laboratory guide. 2nd ed. Harcourt: Brace Jovanovich Publishers; p 279,281. |
| 9. | Patil MB, Jalalpure SS, Pramod HJ. Anti inflammatory activity of Anacardium occidentle leaves. Indian J Pharma Sci 2003:70:110. |
| 10. | Pal D, Panda CO, Datta A, Babu SS, Laha D. Sedative and analgesic activities of the Pet. Ether extract of Cuscuta reflexa Roxb. Stem in mice. Presented in 55 th IPC Chennai: 2003: 245. |
| 11. | Vogel HG, editors. Drug Discovery and Evaluation Pharmacological Assays. 2 nd ed. New York: Springer-Verlager Berlin Heidelberg; 2002. p. 696-7, 692.772. |
Correspondence Address:
Nitin G Sutar
S.N.D. College of Pharmacy, Babhulaon, Tal-Yeola, Dist Nashik - 423 401, Maharashtra
India
  | 2 |
DOI: 10.4103/0973-8258.41182
[Figure 1], [Figure 2]
[Table 1], [Table 2] |
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