International Journal of Green Pharmacy  
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ORIGINAL ARTICLE
Year : 2011  |  Volume : 5  |  Issue : 4  |  Page : 318-324

Qualitative analysis of phytochemicals, and comparative superoxide radical scavenging along with reducing potency of Solanum nigrum using various solvent extracts


1 Department of Biochemistry, SRM Arts and Science College, Chennai, India
2 Department of Biochemistry, DG Vaishnav College of Arts and Science, Chennai, India
3 Faculty of Medicine and Health Science, Universiti Sultan Zainal Abidin, 20400 Kuala Terengganu, Terengganu, Malaysia

Correspondence Address:
U S Mahadeva Rao
PhD., PGDMLT., FICS., Faculty of Medicine and Health Science, Universiti Sultan Zainal Abidin, 20400 Kuala Terengganu, Terengganu
Malaysia
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DOI: 10.4103/0973-8258.94355

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An attempt has been made to screen the phytochemicals, comparative superoxide radical scavenging and reducing potency of Solanum nigrum using various solvent extracts. The herbal powder obtained from plant part-dry leaves were extracted with various solvents. The extracts were analysed for phytochemicals and antioxidants-carotenoids, ascorbic acid, tocopherol, total phenol, proteins, reducing sugars and sterols. Free radical scavenging capacity was analysed in terms of superoxide radial scavenging assay and reducing power assay. Phytochemical characterization of the different extracts revealed the presence of the phytochemicals-alkaloids, phenols, flavonoids, sterol, saponin glycosides, reducing sugars, proteins, cardio active aglycones and cardinolides. Excellent Superoxide Radical scavenging ability found in almost all extracts of S. nigrum. In the present study superoxide radical reduces nitro blue tetrazolium (NBT) to a blue coloured formazan that is measured at 560 nm. Antioxidant activity has been reported to be concomitant with development of reducing power. This shows that extracts might contain reductones like ascorbic acid, reducing sugar, thiol group containing protein which could react with free radicals to stabilize and terminate radical chain reaction. These findings suggest that the promising phytonutrients of the plant could be exploited against oxidative stress, cancer, ageing, Ischemic heart disease in dissolving thrombus, microbial infections and hormone replacement therapy (HRT) justifying their use in traditional medicine as nutraceuticals.


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