Antibacterial activity of plants used in Indian herbal medicine

Pavithra P. S., Janani V. S., Charumathi K. H., Indumathy R., Sirisha Potala, Rama S. Verma


Delonix elata, Enicostemma axillare, Merremia tridentata, Mollugo cerviana and Solanum incanum are medicinal plants used in traditional Indian medicine for the treatment of various ailments. These plants were selected to evaluate their potential antibacterial activity. To determine antibacterial activity and phytochemicals in the crude extracts of five medicinal plants used in traditional Indian medicine for the treatment of various ailments like rheumatism, piles fever, skin diseases and snake bite. The antibacterial activity of organic solvent extracts of these plants were determined by disc diffusion and broth dilution techniques against grampositive bacterial strains (Bacillus subtilis, Staphylococcus aureus) and gram-negative bacterial strains (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa). Results revealed that the chloroform and methanol extracts of D. elata and methanol
extracts of M. cerviana exhibited significant antibacterial activity against gram-positive and gram-negative strains with minimum
bactericidal concentration (MBC) ranging from 1.5 to 100 mg/ml. Methanol extracts of M. tridentata exhibited activity only against
gram-positive bacterial strains with MBC ranging from 12.5 to 100 mg/ml. Extracts of E. axillare and S. incanum showed activity
only against B. subtilis and were not bactericidal at 100 mg/ml. The most susceptible organism to the organic extracts from all the studied plants was B. subtilis and the most resistant organism was P. aeruginosa. The presence of phytochemicals such as alkaloids, tannins, triterpenoids, steroids and glycosides in the extracts of these plants supports their traditional uses as medicinal plants for the treatment of various ailments. The present study reveals potential use of these plants for developing new antibacterial compounds
against pathogenic microorganisms.
Key words: Antibacterial, Enicostemma axillare, Merremia tridentata, Mollugo cerviana, Solanum incanum

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Sujatha S. Complementary and alternative therapies in palliative

care: A transition from modern medicine to traditional medicine

in India. J Cancer Pain Symptom Palliation 2005;1:25-9..

Colombo ML, Bosisio E. Pharmacological activities of Chelidomium

majus L (Paveraceae). Pharmacol Res 1996;33:127-34.

Iwu MM, Duncan AR, Okunji CO. New Antimicrobials of Plant

Origin. In: Janick J,editor. Perspectives on new crops and new uses.

Alexandria: Ashs Press; 1999. p. 457-62.

Thirugnanam S. Mooligai Maruthuvam. (Tamil) Trichy: Selvi

Publishers; 2003. p. 33, 117, 131,139,147.

Compendium of Medicinal Plants. Delhi: National Institute of

Industrial Research (NIIR) publication; 2005. p. 91.

Sethuraman MG, Sulochana N. The anti-inflammatory activity of

Delonix elata. Curr Sci 1986;55:343.

Jayvir A, Minoo P, Gauri B, Raipal K. Nature heals - A Glossary of

Selected Indigenous Medicinal Plants of India. Ahamdabad: Sristi

Innovations; 1997. p. 25, 27.

Sadique J, Chandra T, Thenmozhi V, Elango V. The antiinflammatory

activity of Enicostemma littorale and Mollugo cerviana.

Biochem Med Metab Biol 1987;37:167-76.

Krithikar KR, Basu BD. Indian Medicinal Plants In: Blatter E, Caius

JF, Bhaskar KS, editors. Vol 1-5. Bishen Singh, Mahendra Pal Singh.

Dehradun: 1991. p. 1734.

Klaus H, Nyman U, Smitt UW, Adsersen A, Gudiksen L,

Sreedharan R, Pushpangadan P. In vitro screening of traditional

medicines for anti-hypertensive effect based on inhibition of

the angiotensin converting enzyme (ACE). J Ethnopharmacol


Hatapakki BC, Hukkeri VI, Patil DN, Chavan MJ. Wound healing

activity of aerial parts of Merremia tridentate. Indian Drugs


Britto SJ, Senthilkumar S. Antibacterial activity of Solanum incanum

L leaf extracts. Asian J Microbiol Biotechnol Environ Exp Sci


Mohammed-Al Fatimi, Martina W, Gudrun S, Hansen UL.

Antioxidant, antimicrobial and cytotoxic activities of selected

medicinal plants from Yemen. J Ethnopharmacol 2007;3:657-66.

Harborne JB. Phytochemical methods. London: Chapman and

Hall Ltd; 1996. p. 52-105.

Rios JL, Recio MC, Villar A. Screening methods for natural

products with antimicrobial activity: A review of the literature. J

Ethnopharmacol 1988;23:127-49.

Kone WM, Kamanzi AK, Terreaux C, Hostettmann K, Traore D,

Dosso M. Traditional medicine in north Côte-d'Ivoire: screening

of 50 medicinal plants for antibacterial activity. J Ethnopharmacol


Chattopadhyay RR, Bhattacharyya SK, Medda C, Chanda S,

Datta S, Pal NK. Antibacterial activity of black myrobalan (Fruit

of Terminalia chebula Retz.) against uropathogen Escherichia coli.

Phcog Mag 2007;11:212-5.

Cutler NRC, Sramek, John JS, Prem KN. Pharmacodynamics and

drug development: Perspectives in clinical pharmacology. New

York: John Wiley and Sons; 1994. p. 318.

Farnsworth AC. The role of ethnopharmacology drug development

from plants. England Ciba: John Wiley and Sons; 1982. p. 2-10.

De N, Ifeoma E. Antimicrobial effects of components of the bark

extract of neem Azadirachta indica A. Juss Technol Dev 2002;8:23-8.

Lino A, Degracious O. The in vitro antibacterial activity of Annona

senegalensis, Securidacca longipendiculata and Steganotaenia araliaceae-

Ugandan medicinal plants. Afr Health Sci 2006;6:31-5.

Srinivasan K, Abdul Nazar Dheen M, Perumal G, Mohanasundari

C, Natrajan D. Screening of methanolic leaf extracts of some

medicinal plants against pathogenic bacteria. Adv Plant Sci


Zuin VG, Vilegas JH. Pesticide residue in medicinal plants and

phytomedicines. Phytother Res 2002;14:73-88.

Rios JL, Recio MC. Medicinal plant and antimicrobial activity. J

Ethnopharmacol 2005;100:80-4.

Rios JL, Recio MC, Manez S, Giner RM. Natural triterpenoids as

anti- inflammatory agents. In: Atta-ur- Rahman, editor. Studies in

Natural Products Chemistry. Elsevier; 2000. p. 93-143.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol

Rev 1999;12:564-82.

Hu Fenglina, Lu Ruilia, Huang Baoa, Ming Liangb. Free radical

scavenging activity of extracts prepared from fresh leaves of

selected Chinese medicinal plants. Fitoterapia 2004;75:14-23.

Palombo EA. Phytochemicals from traditional medicinal plants

used in the treatment of diarrhoea: Modes of action and effects

on intestinal function. Phytother Res 2006;20:717-24.

Warrier PK, Longman Staff, Nambiar VP, Ramankutty C,

Vasudevan NR. Indian Medicinal Plants: A Compendium of 500

Species. India: Orient Blackswan; 1996. p. 22.

Johnson T. CRC Ethnobotany Desk Reference. United Kingdom:

CRC Press; 1999. p. 264, 302.

Kalanithi N, Lester P. Micronutrients and Health: Molecular

Biological Mechanisms. The American Oil Chemists Society;

p. 136.

Chhabra SC, Uiso FC, Mshiu EN. Phytochemical screening of

Tanzanian medicinal plants. I. J Ethnopharmacol 1984;11:157-79.

Chhabra SC, Uiso FC, Mshiu EN. Phytochemical Screening of

Tanzanian Medicinal Plants. J of Ethnopharmacol 1984;11:157-79.

Parekh J, Chanda S. In vitro Antimicrobial activity and

phytochemical analysis of some Indian Medicinal Plants. Turk

J Bio 2007;31:53-8.

Nadkarni KM. Dr. K.M. Nadkarni’s Indian Materia Medica:

With Ayurvedic, Unani-tibbi, Siddha, Allopathic, Homeopathic,

Naturopathic and Home Remedies, and Appendices and Indexes.

Mumbai: Popular Prakashan; 1954. p. 485, 804.



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