Hepatoprotective activity studies of herbal formulations

Manisha Bhagwat Kamble, Rahul Kondaji Dumbre, Vinod D. Rangari


Traditional system of medicine recommends various hepatoprotective agents and preparations to treat hepatic disorders. Polyherbal formulations F1 and F2 were developed for treatment of liver disorders by exploiting the knowledge of traditional system of medicine and evaluated for hepatoprotective activity using acute liver toxicity models of CCl4 and Paracetamol induced liver damage in rats. Th e
rats were monitored for morphological changes in liver, biochemical parameter Serum Glutamate Oxaloacetate Transaminase, Serum Glutamate Pyruvate Transaminase, Serum Alkaline Phosphatase, and Serum bilirubin, histopathological studies, and pentobarbitone sleeping time. Both of these formulations F1 and F2 showed signifi cant hepatoprotective activity at dose of 400 mg/kg, which was comparable to silymarin at 6 mg/kg. Formulations F1 and F2 are eff ective both as prophylactic and therapeutic in experimental liver
damage. Biochemical parameters showed better results for formulation F2 but morphological, pentobarbitone sleeping time and hisptopathological observation were similar for both the groups.
Key words: CCl4 induced toxicity, paracetamol induced toxicity, polyherbal formulations, traditional system of medicine

Full Text:



Meyer SA, Kulkarni AP, Hepatotoxicity. In: Hodgson E, Smart RC,

editors. Introduction to biochemical toxicology. 3rd ed. New York:

A. John Wiley and Sons, Inc; 2001. p. 487-90.

Harsh Mohan. Text book of pathology, 4th ed. New Delhi: Jaypee

Publishers; 2002. p. 569-630.

Rege N, Dahanukar S, Karandikar SM. Hepatoprotective eff ect of

Cynidanol (+) against Carbon tetrachloride induced liver damage.

Indian Drugs 1984;22:556-68.

Wealth of India. vol 1, A-Z, PID. New Delhi: CSIR; 1985.

Trivedi N, Rawal VM. Hepatoprotective and Toxicological

evaluation of Andrographis parniculate on severe liver damage.

Indian J Pharmacol 2000;32:288-93.

Mehrotra R, Rawat S, Kulshreshtha DK, Patnaik GK, Dhawan BK.

In vitro studies on the eff ect of certain natural products against

hepatitis B virus. Indian J Med Res 1992;92:133-8.

Handa SS, Sharma A. Hepatoprotective activity of andrographolide

from Andrographis paniculata against carbontetrachloride. Indian J

Med Res 1990;92:276-83.

Chatt opadhyay R, Sarkar SK, Ganguly S, Banerjee RN, Basu TK,

Mukherjee A. Hepatoprotective activity of Azadirachta indica leaves

on paracetamol induced hepatic damage in rats. Indian J Exp Biol


Srimal RC. Turmeric a brief review of medicinal properties.

Fitoterapia 1997;68:483-93.

Sane RT, Chawla JL, Kuber VV. Studies on Phyllanthus amarus-Part

I. Indian Drugs 1997;34:580-4.

Chaudhary K, Singh A, Bagchi GD, Kumar S. Determination

of optimum planting and harvesting schedule for high yields

of Phyllanthus amarus herb containing the hepatoprotective

compound phyllanthin. Indian Drugs 1998;35:481.

Reddy BP, Murthy VM, Venkateshwarlu V, Kokate CK, Rambhau

D. Antihepatotoxic activity of Phyllanthus amarus, Tinospora

cordifolia and Ricinum communis. Indian Drugs 1992;30:338-41.

Ansari RA, Aswal BS, Chander R, Dhawan BN, Garg NK, Kapoor

NK, et al. Hepatoprotective activity of Kutkin-the iridoid glycoside

mixture of Picrorrhiza kurroa. Indian J Med Res 1988;87:401-4.

Saraswat B, Visen PK, Patnaik GK, Dhawan BN. Anticholestatic

eff ect of picroliv, active hepatoprotective principles of Picrorhiza

kurroa against carbontetrachloride induced cholestasis. Indian J

Exp Biol 1993;31:316-8.

Visen PK, Saraswat B, Patnaik GK, Agarwal DP, Dhawan BN.

Protective activity of Picroliv isolated from Picrorrhiza kurroa

against ethanol toxicity isolated rat hepatocytes. Indian J

Pharmacol 1996;28:98-101.

Anand KK, Gupta VN, Rangari VD, Chandan BK. Structure and

hepatoprotective activity of a biß avonoid from Canarium manii.

Planta Med 1992;58:493-5.

Reitzman S, Frankel S. A colorimetric methiod for determination of

serumglutamic oxaloacetic acid glutamic pyruvate transaminases.

Am J Clin Pathol 1957;28:56-63.

Bessey OA, Lowery DM, Brock MJ, A method for rapid

determination of alkaline phosphatase with Þ ve cubic millimeters

of serum. J Biol Chem 1964;164:321-9.

Malloy HT, Evelyn KA. The determination of bilirubin with

photoelectric colorimeter. J Biol Chem 1936;119:481-90.

Seakins A, Robinson DS, The eff ect of the administration of carbon

tetrachloride on the formation of plasma lipoproteins in the rats.

Biochem J 1963;86:401-7.

Portmann B, Talbot IC, Day DW, Davidson AR. Histopathological

changes in the liver following a paracetamol overdose: Correlation

with clinical and Biochemical parameters. J Pathol 1975;117:


DOI: https://doi.org/10.22377/ijgp.v2i3.20


  • There are currently no refbacks.