Evaluation of hypoglycaemic and anti‑hyperglycaemic activities of Guduchi Ghana in Swiss albino mice

Rohit Sharma, Vijay Kumar, B. K. Ashok, R. Galib, P. K. Prajapati, B. Ravishankar


Background: Diabetes mellitus is a common endocrine disorder, characterized by hyperglycaemia resulting from defects in insulin
secretion, insulin action or both. Ayurvedic herbs are relatively low cost, more suitable and have negligible side‑effects than synthetic oral anti‑hyperglycaemic agents. Guduchi is reported as highly potent anti‑diabetic herb in Ayurveda and Guduchi Ghanavati is popularly known by the Ayurvedic fraternity for its therapeutic properties on Madhumeha represented as diabetes mellitus.
Aim: The aim of this study is to evaluate hypoglycaemic and anti‑hyperglycaemic activities of Guduchi Ghana (GG) in Swiss albino mice. Materials and Methods: Hypoglycaemic and anti‑hyperglycaemic potential of GG was evaluated in normal mice using both 18 h fasted mice model and oral glucose tolerance test. GG was suspended in distilled water and administered to animals at the dose of 130 mg/kg. Statistical Analysis: The results were statistically interpreted using Student’s ‘t’‑test for paired and unpaired data to assess the statistical significance and the significant level was set at P < 0.05. Results: GG showed mild reduction in blood sugar
level (BSL) at all the time intervals in normoglycaemic mice. In anti‑hyperglycaemic activity, glibenclamide at a dose of 0.65 mg/kg studied as the reference standard to compare the potency of test drug. Administration of GG prior to glucose over load resulted significant attenuation in BSL at 60 min, 90 min and 120 min in comparison to glucose control group. Conclusions: GG has mild hypoglycaemic and significant anti‑hyperglycaemic activity and can be used in the treatment of diabetes mellitus as well as a supportive drug without fear of producing hypoglycaemia.
Key words: Anti‑hyperglycaemic, Ghana, glibenclamide, Guduchi, hypoglycaemic, tinospora cordifolia

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James R, Mayer B, Ralph A, Allan D, Steven G, Saul G, et al.. Expert

Committee on the Diagnosis and Classification of Diabetes Mellitus.

Report of the expert committee on the diagnosis and classification

of diabetes mellitus. Diabetes Care 2003;26 Suppl 1:S5‑20.

Pavana P, Sethupathy S, Manoharan S. Antihyperglycemic and

antilipidperoxidative effects of Tephrosia purpurea seed extract

in streptozotocin induced diabetic rats. Indian J Clin Biochem


Mandlik RV, Desai SK, Naik SR, Sharma G, Kohli RK. Antidiabetic

activity of a polyherbal formulation (DRF/AY/5001). Indian J Exp

Biol 2008;46:599‑606.

Goel HC, Prem Kumar I, Rana SV. Free radical scavenging

and metal chelation by Tinospora cordifolia, a possible role in

radioprotection. Indian J Exp Biol 2002;40:727‑34.

Subramanian M, Chintalwar GJ, Chattopadhyay S. Antioxidant

properties of a Tinospora cordifolia polysaccharide against

iron‑mediated lipid damage and gamma‑ray induced protein

damage. Redox Rep 2002;7:137‑43.

Gupta SS, Verma SC, Garg VP, Rai M. Anti‑diabetic effects of

Tinospora cardifolia. I. Effect on fasting blood sugar level, glucose

tolerance and adrenaline induced hyperglycaemia. Indian J Med

Res 1967;55:733‑45.

Sinha K, Mishra NP, Singh J, Khanuja SP. Tinospora

cordifolia (Guduchi), a reservoir plant for therapeutic applications:

A review. Indian J Tradit Knowl 2004;3:257‑70.

Grover JK, Vats V, Rathi SS. Anti‑hyperglycemic effect of Eugenia

jambolana and Tinospora cordifolia in experimental diabetes and

their effects on key metabolic enzymes involved in carbohydrate

metabolism. J Ethnopharmacol 2000;73:461‑70.

Stanely P, Prince M, Menon VP. Hypoglycaemic and other related

actions of Tinospora cordifolia roots in alloxan‑induced diabetic rats.

J Ethnopharmacol 2000;70:9‑15.

Stanely Mainzen Prince P, Menon VP. Hypoglycaemic and

hypolipidaemic action of alcohol extract of Tinospora cordifolia roots

in chemical induced diabetes in rats. Phytother Res 2003;17:410‑3.

Kar A, Choudhary BK, Bandyopadhyay NG. Comparative

evaluation of hypoglycaemic activity of some Indian medicinal

plants in alloxan diabetic rats. J Ethnopharmacol 2003;84:105‑8.

Rathi SS, Grover JK, Vikrant V, Biswas NR. Prevention of

experimental diabetic cataract by Indian Ayurvedic plant extracts.

Phytother Res 2002;16:774‑7.

Dhulia I, Parcha V, Pant G, Kumar D, Maithani A. Antihyperglycemic

effect of Methanolic extract of Tinospora cordifolia (Willd.) stem on

experimentally induced diabetic rats. J Pharm Res 2011;4:2828‑30.

Rajalakshmi M, Eliza J, Priya CE, Nirmala A, Daisy P.

Antidiabetic properties of Tinospora cordifolia stem extracts on

streptozotocin‑induced diabetic rats. Afr J Pharm Pharmacol


Rajalakshmi M, Eliza J, Priya CE, Nirmala A, Daisy P.

Antidiabetic properties of Tinospora cordifolia stem extracts on

streptozotocin‑induced diabetic rats. Afr J Pharm Pharmacol


Wadood N, Wadood A, Shah SA. Effect of Tinospora cordifolia on

blood glucose and total lipid levels of normal and alloxan‑diabetic

rabbits. Planta Med 1992;58:131‑6.

Grover JK, Rathi SS, Vats V. Amelioration of experimental diabetic

neuropathy and gastropathy in rats following oral administration

of plant (Eugenia jambolana, Mucuna pruriens and Tinospora cordifolia)

extracts. Indian J Exp Biol 2002;40:273‑6.

Acharya YT. Siddha Yoga Sangraha. Jwaradhikara, 13th ed. Nagpur:

Baidyanath Ayurveda Bhavan Ltd; 2008. p. 4.

Anonymous. Quality Standards of Indian Medicinal Plants. Vol. 1.

New Delhi: ICMR; 2003. p. 212.

Parashar R, edi t o r . Sharangadhara Samhi t a of

Sharangadhara (Madhyamakhanda). 4th ed., Ch. 2, Verse 1‑2.

Nagpur: Baidyanath Ayurveda Bhavan Ltd.; 1994. p. 189.

Sharma R. The effect of two different dosage forms of

Guduchi, i.e., Satva and Ghana W.S.R. Antihyperglycemic

effect on Madhumeha (NIDDM). M. D. Dissertation. Jamnagar:

Dept. R.S. and B.K., I.P.G.T. and R.A; 2012.

Paget GE, Barnes JM. Evaluation of drug activities. In:

Lawrence DR, Bacharach AL, editors. Pharmacometrics. Vol. 1.

New York: Academic Press; 1964. p. 161.

Pilkhwal SS, Sah ML, Juyal V, Pandey S. Hypoglycemic activity of

aqueous extract of Urtica parviflora roxb. in normoglycemic rats.

Int J Phytomedicine 2010;2:47‑51.

Available from: http://en.wikipedia.org/wiki/Hypoglycemia. [Last

accessed on 2013 Feb 17].

Puranik N, Kammar KF, Sheela D. Anti‑diabetic activity of

Tinospora cordifolia (Willd.) in streptozotocin diabetic rats; does it

act like sulfonylureas? Turk J Med Sci 2010;40:265‑70.

Wadkar KA, Magdum CS, Patil SS, Naikwade NS. Anti‑diabetic

potential and Indian medicinal plants. J Herb Med Toxicol


DOI: http://dx.doi.org/10.22377/ijgp.v7i2.313


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