Comparative hepatoprotective activity of Liv-52 and livomyn against carbon tetrachloride-induced hepatic injury in rats

V.D. Sapakal, R.V. Ghadge, R.S. Adnaik, N.S. Naikwade, C.S. Magdum


Liver disease is one of the serious health problems. Herbs play a major role in the management of various liver disorders. Th e present study was conducted to compare the hepatoprotective activity of two marketed formulations, Liv-52 and Livomyn. Th e formulations showed signifi cant hepatoprotective eff ect by reducing elevated serum enzyme levels such as glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT), bilirubin content (direct and total) and total protein. Th ese biochemical observations
were supplemented by weight and histopathological examination of liver sections. Various pathological changes such as steatosis,
centrilobular necrosis and vacuolization observed in rats treated only with CCl4, but the groups treated with the CCl4, and hepatoprotective formulations were producted to a moderete extent from such pathological changes. Silymarin was used for positive control. It was concluded from study that Liv-52 has shown more signifi cant hepatoprotective activity against c CCL4-induced hepatotoxicity in rats in comparison to Livomyn.
Key words: Carbon tetrachloride, hepatoprotective activity, Liv-52, livomyn, silymarin

Full Text:



Reddy PB, Reddy CK, Rambhau D, Venkateshvarala V, Murthy VN.

Anti hepatoprotective activity of some Ayurvedic preparations.

Indian J Pharm Sci 1993;55:137-40.

Gonzalez FJ. The molecular biology of cytochrome P450s.

Pharmacol Rev 1988;40:243-88.

Mac Cay PB, Lai EK, Poyer JL. Oxygen and carbon center free

radical formation during carbon tetrachloride metabolism

observation of lipid radical in-vivo and in-vitro. J Biol Chem


Poli G, Chiarpott o E, Albano E, Cotalasso D, Nanni G, Marinari

UM. Carbon tetrachloride induced inhibition of hepatocyte

lipoprotein secreation: Functional impairment of Golgi apparatus

in the early phases of such injury. Life Sci 1985;36:533-9.

Karandikar SM, Jnglckar GV, Chitale GK, Balwani JH. Protection

by indigenous drugs against hepatotoxic effects of carbon

tetrachloride: A long term study. Acta Pharmacol et Toxicol


Saini NR, Saini N. Liv-52 protection against radiation induced

lesions in mammalian liver. Radiobiol Radiotber 1985;26:279-82.

Dhumal MS, Mane IH, Patel SS. Effect of Liv-52 on carbon

tetrachloride. Indian J Pharmacol 1989;21:96-9.

Ghosh MN. Fundamentals of Experimental Pharmacology. 3rd ed.

Kolkatt a: Hilton and Co; 2005. p. 192.

Jindal MN, Patel VK, Patel NB. Some pharmacological activities of

aqueous and alcoholic extracts of Cichorium intybus Linn. Indian J

Pharmacol 1975;7:104.

Gilani, AH, Janbaz KH. Evaluation of liver protective potential of

Cichorium intybus seed extract on acetaminophen and CCl4-induced

damage. Phytomed 1994;1:193-7.

Sultana S, Shahid P, Mohammad I, Mohammad A. Crude extracts

of hepatoprotective plants, Solanum nigrum and Cichorium intybus

inhibits Free radical mediated DNA damage. J Ethnopharmacol


Manoj B, Aqueed UK. Protective role of Lawsonia alba Lam against

CCl4 induced hepatic damage in albino rats. Indian J Exp Biol


Gennaro AR. Remington: The Science and Practice of Pharmacy.

th ed. Vol. 1, Easton PA: MacPublishing Co; 1995. p. 111.

Mukherjee PK. Quality control of herbal drugs: An approach to

evaluation of botanicals. business horizons. New Delhi, India:

p. 518-98.

Mukherjee PK. Plant products with hypochlesterolemic potentials.

In: Taylor Steve L, editor. vol 47. Elsevier Science, USA: Advanced

in Food and Nutrition Research; 2003. p. 277-338.

Drotman RB, Lawhorn GT. Serum enzymes as indicators of chemical

induced liver damage. Drug Chem Toxicol 1978;1:163-71.

Reddy BP, Murthy VN, Venkateshwarlu V, Kokate CK, Rambhau D.

Antihepatotoxic activity of Phyllanthusniruri, Tinuspora cordifolia

and Ricinus communis. Indian Drugs 1993;30:338-41.

Johnson E, Krocning C. Mechanism of early CCl4 toxicity in

cultured rat hepatocytes. Pharmacol Toxicol 1998;83:231-9.

Cotran S, Kumar V, Robbins SL. Cell injury and cellular death. In:

Robbins Pathologic Basic of Disease. 5th ed. Prism Book Pvt. Ltd;

p. 379-430.

Kaplowitz N, Aw TY, Simon FR, Stolz A. Drug induced

hepatotoxicity. Ann Int Med 1986;104:826-39.

Srivastava SP, Chen NO, Holtzman JC. The In vitro NADPH

dependent inhibition by CCl4 of the ATP-dependent calcium

uptake of hepatic microsomes from male rats: Studies on the

mechanism of inactivation of the hepatic microsomes calcium

pump by the CCl4 radical. J Biol Chem 1990;265:8392-9.

Shenoy AK, Somayaji SN, Bairy KC. Hepatoprotective eff ects of

Ginkgo Biloba against CCl4 induced hepatic injury in rat. Indian

J Pharmacol 2001;33:260-6.



  • There are currently no refbacks.