Metabolite profiling and antioxidant activity of leaves, stem, and flowers of Tridax procumbens: A medicinal herb

Abbas Ali Mahdi


Introduction: This study was designed to analyze in vitro antioxidant activity and estimate the metabolic profiling of
non-polar and polar extracts of Tridax procumbens leaves stem and flowers. Materials and Methods: Antioxidant
activity of hexane and methanol extract was determined through DPPH free-radical scavenging assay and nitric
oxide assay using the standard method and metabolite profiling of non-polar and polar extracts of T. procumbens
was done by using gas chromatography-Mass spectrometry (GC-MS). Results: The hexane extract of
T. procumbens flower (HTPF) showed the most significant antioxidant activity concerning the methanolic extract,
which is indicated by its IC50 values obtained from the DPPH was 33.14 μg/mL and 34.52 μg/mL (HTPF and
MTPL) wherein IC50 for nitric oxide assay was 16.74 and 34.26 μg/mL (HTPF and MTPL), respectively. In
addition, GC-MS-based metabolic profiling of T. procumbens parts (leaves, stem, and flowers) showed hundred
and more chemically diverse metabolites. A very high concentration of oleanolic acid was found in hexane leaves
(32.13 ± 0.3.11%) and flowers (17.66 ± 1.71%) extract while in stems (12.63 ± 1.22). The other metabolites
such as 2, 4-di-tert-butyl phenol, and germacrene were identified as major metabolites. Trans-farnesol and
two major fatty acids palmitic and linolenic and high percent peak area of stearic acid were detected in stems.
Discussion and Conclusion: GC-MS analysis showed several compounds with, high content of terpenes and
sterols which may be a reason for their antioxidant activity. The antioxidant property of medicinal plants has a
different role in combating various diseases.

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