Evaluation of antibacterial and antifungal activities of olive (Olea europaea) essential oil

R. K. Upadhyay

Abstract


Context: Essential oil Olea europaea was investigated for its antibacterial and antifungal activities. Aim: To evaluate antimicrobial activity of O. europaea essential oil against infectious microbial pathogens. Settings and Design: Seeds of O. europaea were grounded by using domestic mixer and powdered material was hydro‑distilled in Clevenger apparatus continuously for 5 hrs to yield essential oil. Essential oil was analysed on Gas‑Chromatography‑Mass spectrometry (GC‑MS) from which 24 components were identified, representing total
99.98% of the oil. Extracted oil was evaluated for their antibacterial and antifungal activities. Materials and Methods: Paper disc diffusion and serial micro‑dilution assays were performed for the determination of inhibition zone diameters and minimal inhibitory concentration, respectively.

Results: The O. europaea essential oil showed the diameter of inhibition zone (DIZ) ranging from 19.4 ± 0.07‑26.4 ± 0.09 mm
at a concentration level of 28 μg/disc (W/V) separately in all the ten strains tested. The minimum inhibitory concentration of essential oil against bacterial strains was obtained in a range of 7.0-56.0 μg/ml while in and fungal strains it was in a range of 7.0‑28 μg/ml. Statistical analysis: All statistical calculations are expressed as mean ± SE of three replicates. Data were analyzed by one‑way Analysis of Variance (ANOVA) to locate significant variations in oil activity in various bacterial and fungal strains followed by the Duncan’s multiple range tests. Conclusions: Antibacterial and antifungal activities of O. europaea essential oil are due to the presence of certain secondary
plant metabolites such as terpenoids, steroids and flavonoids, esters, and acids, which were identified in the essential oil. The oil components can be further investigated for their biological activities and study to overcome the problem of drug resistance in microbes.
Key words: Antimicrobial activity, essential oil, gas-chromatography-mass spectrometry analysis, inhibition zone diameters, minimum bacterial concentration, minimum fungicidal concentration, minimum inhibitory concentration, Olea europaea


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References


Ouattara B, Simard RE, Holley RA, Pictte GJ, Begin A. Antibacterial

activity of selected fatty acids and essential oils against six meat

spoilage organism. Int J Food Microbiol 1997;37:155‑62.

Calsamiglia S, Castillejos L, Busquet M. Alternative to antimicrobial

growth promoters in cattle. In: Garnsworthy PC, Wiseman J,

editors. Nottingham. Recent Advances in Animal nutrition.

Nottingham: University Press; 2006. p. 129‑167.

Burt SA, Vlielander R, Haagsman HP, Veldhuizen EJ. Increase in

activity of essential oil components carvacrol and thymol against

Escherichia coli O157: H7 by addition of food stablizers. J Food Prot

;68:919‑26.

Mimica‑Dukic N, Bozin B, Sokovic M, Simin N. Antimicrobial and

antioxidant activities of Melissa officinalis L. (Lamiaceae) essential

oil. J Agric Food Chem 2004;52:2485‑9.

Sousa A, Ferreira IC, Calhelha R, Andrade PB, Valentao P, Seabra R,

et al. Phenolics and antimicrobial activity of traditional stoned table

olives ‘alcaparra’. Bioorg Med Chem 2006;14:8533‑8.

Sylvestre M, Pichette A, Longtin A, Nagau F, Legault J. Essential

oil analysis and anticancer activity of leaf essential oil of Croton

flavens L. from Guadeloupe. J Ethnopharmcol 2006;103:99‑102.

Cosentino S, Barra A, Pisano B, Cabizza M, Pirisi FM, Palmas F.

Composition and antimicrobial properties of Sardinian

Juniperus essential oil against foodborn pathogens and spoilage

microorganism. J Food Prot 2003;66:1288‑91.

Jones NL, Shabib S, Sherman PM. Capsaicins as an inhibitor of the

growth of the gastric pathogen Helicobactor pylori. FEMS Microbiol

Lett 1997;146:223‑7.

Lawless J. The illustrated Encyclopedia of essential oils. The

Complete Guide to the Use of Oils in Aromatherapy and

Herbalism, Shaftesbury, Dorset, England: Elements Book Ltd;

p. 256.

Mishra AK, Dubey NK. Evaluation of essential oils for their toxicity

against fungi causing deterioration of stored food commodities.

App Environ Microbiol 1994;60:1101‑5.

Faid M, Bakhy K, Anchad M, Tantaoui‑Elaraki A, Alo mondpaste.

Physiochemical and microbiological characterizations and

preservation with scorbic acid and cinnamon. J Food Prot

;58:541‑50.

Buttner MP, Willeke K, Grinshpun SA. Sampling and analysis

of airborne microorganisms in manual of environmental

microbiology. In: Hurst CJ, Mclnerney GR, Stetzenbach MJ,

Walter LD, editors. Washington: American Society of Microbiology;

p. 629‑640.

Guenther E. The production of essential oils: Methods of

distillation, enfleurage, maceration and extraction with volatile

solvent. In: Guenther E, editor. The essential oils. History‑origin

in plants. production analysis. Vol. 1:85‑188. Krieger Publ. Co.,

Malabar, FL.

Reynolds JE. Martindale, The Extra Pharmacopoeia. 31st ed. London,

Great Britain: Royal Pharmaceutical Society; 1996:1181‑1182, 1741.

Lis‑Balchin M, Deans SG, Eaglesham E. Relationship between

bioactivity and chemical composition of commerical essential oils.

Flavour Fragr J 1998;13:98‑104.

Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro

antibacterial activity of some plant essential oils. BMC Complement

Altern Med 2006;6:39.

Medina E, de Castro A, Romero C, Brenes M. Comparison of the

concentrations of phenolic compounds in olive oils and other plant

oils: Correlation with antimicrobial activity. J Agric Food Chem

;54:4954‑61.

Ozcan MM, Sagdic O, Ozkan G. Inhibitory effects of spice essential

oils on the growth of Bacillus species. J Med Food 2006;9:418‑21.

Brady A, Loughlin R, Gilipin D, Kearney P, Tunney M. In

vitro activity of tea‑tree oil against clinical skin isolates of

methicillin‑resistant and sensitive Staphylococcus aureus and

coagulase‑negative staphylococci growing planktonically and as

biofilms. J Med Microbiol 2006;55:1375‑80.

Ferrini AM, Mannoni V, Aureli P, Salvatore G, Piccirilli E,

Ceddia T, et al. Melaleuca alternifolia essential oil possesses potent

anti‑staphylococcal activity extended to strains resistant to

antibiotic. Int J Immunopathol Pharmacol 2006;19:539‑44.

Raybaudi‑Massilia RM, Mosqueda‑Melgar J, Martin‑Belloso O.

Antimicrobial activity of essential oils on Salmonella enteritidis,

Escherichia coli, and Listeria innocua in fruit juices. J Food Prot

;69:1579‑86.

O’ Gara EA, Hill DJ, Maslin DJ. Activities of garlic oil, garlic

powder and their diallyl constituents against Helicobacter pylori.

Appl Environ Microbiol 2000;66:2269‑73.

Brenes M, Garcia A, Garcia P, Rios JJ, Garrido A. Phenolic compounds

in Spanish olive oils. J Agric Food Chem 1999;47:3535‑40.

Shin S, Kim JH. In vitro inhibitory activities of essential oils from

two Korean thymus species against antibiotic‑resistant pathogens.

Arch Pharm Res 2005;28:897‑901.

Liu XT, Shi Y, Yu B, Williams ID, Sung HH, Zhang O, et al.

Antibacterial diterpenoids from Sagittaria pygmaea. Planta Med

;73:84‑90.

Botelho MA, Ngueiro NA, Bastos GM, Fonseca SG, Lemos TL,

Matos FJ, et al. Antimicrobial activity of the essential oil from Lippia

sidoides, carvacrol and thymol against oral pathogens. Braz J Med

Biol Res 2007;40:349‑56.

Pepeljnjak S, Kosalec I, Kalodera Z, Blazevic N. Antimicrobial

activity of juniper berry essential oil (Juniperus communis L.,

Cupressaceae). Acta Pharm 2005;55:417‑22.

Kizil G, Toker Z, Ozen HC, Aytekin C. The antimicrobial activity

of essential oils of Hypericum scabrum, Hypericum scabroides and

Hypericum triquetrifolium. Escherichia coli O 157:H7 by addition of

food stabilizers. J Food Prot 2004;68:919‑26.

Lo Cantore P, Iacobellis NS, De Marco A, Capasso F, Senatore F.

Antibacterial activity of Coriandrum sativum L Foeniculum vulgare

Miller Var. vulgare (Miller) essential oils. J Agric Food Chem

;52:7862‑6.

Chang ST, Chen PF, Chang SC. Antibacterial activity of leaf

essential oil and their constituents from Cinnamomum osmophloeum.

J Ethopharmacol 2001;77:123‑7.

Lee SB, Cha KH, Kim SN, Altantsetseg S, Shatar S, Sarangerel O,

et al. The antimicrobial activity of essential oil from Dracocephalum foetidum against pathogenic microorganisms. J Micobiol

;45:53‑7.

Lamber RJ, Skandamis PN, Coote PJ, Nycas GJ. A study of the

minimum inhibitory concentration and mode of action of oregano

essential oil, thymol and carvacrol. J Appl Microbiol 2001;91:453‑62.

Walsh SE, Maillard JY, Russell AD, Catrenich CE, Charbonneau DL,

Bartolo RJ. Activity and mechanism of action of selected biocidal

agents on Gram‑positive and ‑negative bacteria. J Appl Microbiol

;94:240‑7.

Upadhyay RK, Dwivedi P, Ahmad S. Antifungal activity of 16 plant

essential oils against S. cerevisiae, Rhizopus stolonifer and Aspergillus

flavus. J Phar Res 2011;4:1153‑6.

Upadhyay RK, Dwivedi P, Ahmad S. Screening of antibacterial

activity of six plant essential oils against pathogenic bacterial

strains. Asian J Med Sci 2010;2:152‑8.

Upadhyay RK, Ahmad S, Tripathi R, Rohtagi L, Jain SC.

Screening of antimicrobial potential of extracts and pure

compounds isolated from Capparis decidua. J Med Plants Res

;4:439‑45.




DOI: https://doi.org/10.22377/ijgp.v8i3.382

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