ORIGINAL ARTICLE
Year : 2009 | Volume
: 3 | Issue : 1 | Page : 47--51
Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea ( Camellia sinensis ) granules
H Misra1, D Mehta2, BK Mehta2, M Soni3, DC Jain1
1 School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain - 456 010; Green Technology Department, Ipca Laboratories Limited, Ratlam - 457 002, India
2 School of Studies in Chemistry and Biochemistry, Vikram University, Ujjain - 456 010, India
3 Mandsaur Institute of Pharmacy, Mandsaur - 458 001, Madhya Pradesh, India
Correspondence Address:
D C Jain
Green Technology Department, Ipca Laboratories Limited, Ratlam - 457 002, Madhya Pradesh
India
A simple, precise and accurate high-performance thin-layer chromatographic (HPTLC) method has been established and validated for screening and quantitative estimation of caffeine in different extracts of tea samples ( Camellia sinenesis ) . Separation was performed on silica gel 60 F 254 HPTLC plates with ethyl acetate:methanol in the proportion of 27: 3 ( v/v ), as a mobile phase. The determination was carried out in the ultraviolet (UV) region using the densitometric remission-absorbance mode at 274nm. Maximum recovery of caffeine was achieved when extracted with 5% diethyl amine in DM water (v/v). The maximum concentration of caffeine in tea samples was found to be 2.145%, dry weight basis. Caffeine response was found to be linear over the range of 2-14µg per zone. Limits of detection and quantitation were found to be 40 and 120ng/spot, respectively. The HPTLC method was validated in terms of precision, accuracy, sensitivity and robustness. Some rare parameters for the HPTLC method like calculation of flow constant (k) and plate efficiency (N) are included specially.
How to cite this article:
Misra H, Mehta D, Mehta B K, Soni M, Jain D C. Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea ( Camellia sinensis ) granules.Int J Green Pharm 2009;3:47-51
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How to cite this URL:
Misra H, Mehta D, Mehta B K, Soni M, Jain D C. Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea ( Camellia sinensis ) granules. Int J Green Pharm [serial online] 2009 [cited 2013 May 25 ];3:47-51
Available from: http://www.greenpharmacy.info/article.asp?issn=0973-8258;year=2009;volume=3;issue=1;spage=47;epage=51;aulast=Misra;type=0 |
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