Pharmacognostical review of Urtica dioica L.

Bhuwan Chandra Joshi, Minky Mukhija, Ajudhia Nath Kalia


Urtica dioica L. belongs to the family Urticaceae, is a perennial herb commonly known as ‘stinging nettle’. This herb is found in many South Asian Countries, Indian subcontinent and has been known in the world as a medicinal herb for a long time. U. dioica is widely used by the traditional medicinal practitioners for curing various diseases such as nephritis, haematuria, jaundice, menorrhagia, arthritis and rheumatism. Phytochemical studies revealed the presence of many valuable chemical compounds like phytosterols, saponins, flavanoids, tannins, proteins and amino acids. The plant also has been used as food, fiber, paint, manure and cosmetics. U. dioica has been reported to have various pharmacological activities like antibacterial, antioxidant, analgesic, anti‑inflammatory, antiviral, immunomodulatory, hepatoprotective, anti‑colitis and anticancer effects. The current review summarizes published information about the ethnopharmacology, phytochemistry, biological activities and toxicological reports of U. dioica. The present review summarizes
all the research work carried out on this plant in order to provide updated information for future works.
Key words: Urtica dioica L., Ethanomedicinal uses, phytochemistry, pharmacology, toxicology

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Krystofova O, Adam V, Babula P, Zehnalek J, Beklova M, Havel L,

et al. Effects of various doses of selenite on stinging nettle (Urtica

dioica L.). Int J Environ Health Res Public Health 2010;7:3804‑15.

CSIR. Wealth of India publications and information directory.

New Delhi: CSIR; 1998. p. 419‑21.

Grieve M. A modern herbal. 3rd ed. London: Tiger Books

International; 1931. p. 912.

Khare CP. Indian Medicinal Plants an Illustrated Dictionary.

New York: Springer Science Business Media LLC; 2007. p. 686‑7.

Chopra RN, Nayar SL, Chopra IC. Supplement to Glossary of

Indian Medicinal Plants. New Delhi: CSIR; 1956. p. 686‑7.

Green T. Universal herbal. 2nd ed. London: Caxton Press; 1824.

p. 729.

Tucakov J. Lecenje biljem‑fitoterpija. Beograd: Rad; 1997. p. 405.

Wetherilt H. Evaluation of urtica species as potential sources of

important nutrients. Dev Food Sci 1992;29:15‑25.

Rafajlovska V, Kavrakovski Z, Simonovska J, Srbinoska M.

Determination of protein and mineral contents in stinging nettle.

Qual Life 2013;4:26‑30.

Kataki MS, Murugamani V, Rajkumari A, Mehra PS, Awasthi D,

Yadav RS. Antioxidant, hepatoprotective and anthelmintic

activities of methanol extract of Urtica dioica L. Leaves. Pharm

Crops 2012;3:38‑46.

Ji TF, Liu CH, Wang AG, Yang JB, Su YL, Yuan L, et al. Studies

on the chemical constituents of Urtica dioica L. grown in Tibet

Autonomous Region. Zhong Yao Cai 2007;30:662‑4.

Shackebaei D, Godini A, Abolghazi M, Majnouni M, Hesari M.

Protection of ischemic and reperfused rat heart by aqueous extract

of urtica dioica. Iran Cardiovasc Res J 2010;4:107‑11.

Mavi A, Terzi Z, Ozgen U, Yildirim A, CoÅŸkun M. Antioxidant

properties of some medicinal plants. Prangos ferulacea (Apiaceae),

Sedum sempervivoides (Crassulaceae), Malvan eglecta (Malvaceae),

Cruciatataurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae),

Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae).

Biol Pharm Bull 2004;7:702‑5.

Gulcin I, Kufrevioglu OI, Oktay M, Buyukokuroglu ME.

Antioxidant, antimicrobial, antiulcer and analgesic activities of

nettle (Urtica dioica L.). J Ethnopharmacol 2004;90:205‑15.

Koch E. Extracts from fruits of saw palmetto (Sabal serrulata) and

roots of stinging nettle (Urtica dioica): Viable alternatives in the

medical treatment of benign prostatic hyperplasia and associated

lower urinary tracts symptoms. Planta Med 2001;67:489‑500.

Hadizadeh I, Peivastegan B, Kolahi M. Antifungal activity of

nettle (Urtica dioica L.), colocynth (Citrullus colocynthis L. Schrad),

oleander (Nerium oleander L.) and konar (Ziziphus spina‑christi L.)

extracts on plants pathogenic fungi. Pak J Biol Sci 2009;12:58‑63.

Kukric ZZ, Topalic‑Trivunovic LN, Kukavica BM, Matos SB,

Pavicic SS, Boroja MM, et al. Characterization of antioxidant and

antimicrobial activities of nettle leaves (Urtica dioica L.). Acta

periodica technologica (APTEFF) 2012;43:259‑72.

Modarresi‑Chahardehi A, Ibrahim D, Fariza‑Sulaiman S,

Mousavi L. Screening antimicrobial activity of various extracts of

Urtica dioica. Rev Biol Trop 2012;60:1567‑76.

Nahata A, Dixit VK. Evaluation of 5α‑reductase inhibitory activity of

certain herbs useful as antiandrogens. Andrologia 2014;46:592‑601.

Zlatko J, Miroslav K, Zorica P. Grain‑protective properties of herbal

extracts against the bean weevil Acanthoscelides obtectus Say. Ind

Crops Prod 2007;26:100‑4.

Akbay P, Basaran AA, Undeger U, Basaran N. In vitro

immunomodulatory activity of flavonoid glycosides from Urtica

dioica. Phytother Res 2003;17:34‑7.

Alisi CS, Emejulu AA, Nwagou LA, Onyema OO. Decreased

cardiovascular risk and resistance to hyperlipemia‑induced hepatic

damage in rats by aqueous extract of Urtica dioica. Afr J Biochem

Res 2008;2:102‑6.

Golalipour J, Khori V. The protective activity of Urtica dioica

leaves on blood glucose concentration and beta‑cells in

streptozotocin‑diabetic rats. Pak J Biol Sci 2007;10:1200‑4.

Tahri A, Yamani S, Legssyer A, Aziz M, Mekhfi H, Bnouham M,

et al. Acute diuretic, natriuretic and hypotensive effects of a

continuous perfusion of aqueous extract of Urtica dioica in the rat.

J Ethnopharmacol 2000;73:95‑100.

Kanter M, Coskun O, Budancamanak M. Hepatoprotective effects

of Nigella sativa L and Urtica dioica Linn. on lipid per oxidation,

antioxidant enzyme systems and liver enzymes in carbon

tetrachloride‑treated rats. World J Gastroenterol 2005;11:6684‑8.

Riehemann K, Behnke B, Schulze‑Osthoff K. Plant extracts from

stinging nettle (Urtica dioica), an anti‑rheumatic remedy, inhibit

the pro‑inflammatory transcription factor NF‑KappaB. FEBS Lett


Sezik E, Yesilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T.

Traditional Medicine in Turkey X. Folk Medicine in Central

Anatolia. J Ethnopharmacol 2001;75:95‑115.

Harrison RK. Healing Herbs of the Bible. Leiden: EJ Brill

Publishers; 1966. p. 58.

Wetherilt H. Nutritional Evaluation of Urtica Species. In:

Kavalali G, editor. Urtica. London and New York: Taylor and

Francis; 2003. p. 84‑92.

Council of Europe, Committee of Experts on Flavorings Substances,

Natural Sources of Flavorings. Report No. 3. Belgium: Council of

Europe Publishing; 2008. p. 208.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Dehradun:

International book distributors; 2008. p. 2340.

Available from: http://www.rain‑

U44JMHKSxXY. [Last accessed on 2014 June 14].

Bisht S, Bhandari S, Bisht NS. Urtica dioica (L): An undervalued,

economically important plant. Agric Sci Res J 2012;2:250‑2.

Jinous A, Mohajerani R. Phytochemistry and pharmacological

properties of Urtica dioica L. J Med Plants Res 2012;6:5714‑9.

Available from: htpp://

StingingNettle.pdf. [Last accessed on 2014 June 14].

Bisset NG. Herbal drugs and phytopharmaceuticals. Boca Raton:

CRC Press; 1994. p. 105.

British herbal pharmacopoeia. London: British Herbal Medicine

Association; 1996.

Khare V, Kushwaha P, Verma S, Gupta A, Srivastava S, Singh

Rawat AK. Pharmacognostical evaluation and antioxidant activity

of Urtica dioica L. Chin Med J 2012;3:128‑35.

Upton R. Stinging nettles leaf (Urtica dioica L.): Extraordinary

vegetable medicine. J Herb Med 2013;3:9‑38.

Gul S, Demirci B, BaÅŸer KH, Akpulat HA, Aksu P. Chemical

composition and in‑vitro cytotoxic, genotoxic effects of essential oil

from Urtica dioica L. Bull Environ Contam Toxicol 2012;88:666‑71.

Bombardelli E, Morazzoni P. Urtica dioica L. Fitoterapia


Ellnain‑Wojtaszek M, Bylka W, Kowalewski Z. Flavanoids

compounds in Urtica dioica L. Herba Pol 1986;32:131‑7.

Kudritsata S, Filman G, Zagorodskaya L, Chikovanii D.

Carotenoids of Urtica dioica. Chem Nat Comp 1986;22:604‑5.

Rafajlovska V, Rizova V, Djarmati Z, Tesevic V, Cvetkov L. Contents

of fatty acids in stinging nettle extracts (Urtica dioica L.) obtained

with supercritical carbon dioxide. Acta Pharm 2001;51:45‑51.

Fu HY, Chen SJ, Chen RF, Ding WH, Kuo‑Huang LL, Huang RN.

Identification of oxalic acid and tartaric acid as major persistent

pain‑inducing toxins in the stinging hairs of the nettle, Urtica

thunbergiana. Ann Bot 2006;98:57‑65.

Czarnetzki BM, Thiele T, Rosenbach T. Immunoreactive

leukotrienes in nettle plants (Urtica urens). Int Arch Allergy

Immunol 1990;91:43‑6.

Collier HO, Chesher GB. Identification of 5‑hydroxytryptamine

in the sting of the nettle (Urtica dioica). Br J Pharmacol Chemother


Bucar F, Britzmann B, Streit B, Weigend M. LC‑PDA‑MS‑profiles

of phenolic compounds in extracts aerial parts of Urtica species.

Planta Med 2006;72:152.

Grevsen K, Frette XC, Christensen LP. Concentration and

composition of flavonol glycosides and phenolic acids in aerial

plants of stinging nettles (Urtica dioica L.) are affected by nitrogen

fertilization and by harvest time. Eur J Hortic Sci 2008;73:20‑7.

Fleming T, Streit B. PDR for Herbal Medicines. 2nd ed. Paris: Tec

and Doc publisher; 2000. p. 729‑95.

Harborne BI, Baxter H, Moss PG. Phytochemical Dictionary. II‑a

ed. New York: Taylor–Francis; 1999. p. 4421, 658, 1664, 1962, 1964,

Wichtl M, Anton R. Plantes therapeutiques. Paris: TEC and DOC;

p. 618.

Kavtaradze NS, Alaniya MD. Anthocyanins glycosides from urtica

dioica. Chem Nat Comp 2003;39:315.

Bertok L. Makyar Allatorvosok Lapja. 1956;11:162.

Frank B, Bohn I, Uehleke B. Urtica. In: Blaschek W, Hansel R,

Keller K, Reichling J, Rmipler H, Schneider G, editors. Vol. 3.

Hagers Hand buch. Berlin: Springer; 1998. p. 710‑36.

Lapke C, Nundel M, Wendel G, Schilcher H, Riedel E.

Concentrations of free amino acid in herbal drugs. Planta Med


Wichtl M, Schafer‑Korting M. Brennessel blatter. Stuttgart:

Wissneschaft Verlagsgesell; 1994. p. 53.

Harput US, Saracoglu I, Ogihara Y. Stimulation of lymphocyte

proliferation and inhibition of nitric oxide production by aqueous

Urtica dioica extract. Phytother Res 2005;19:346‑8.

Bnouham M, Merhfour FZ, Ziyyat A, Mekhfi H, Aziz M,

Legssyer A. Antihyperglycemic activity of the aqueous extract of

Urtica dioica. Fitoterapia 2003;74:677‑81.

Pinelli P, Ieri F, Vignolini P, Bacci L, Baronti S, Romani A. Extraction

and HPLC analysis of phenolic compounds in Leaves, stalks, and

textile fibers of Urtica dioica L. J Agric Food Chem 2008;56:9127‑32.

Rohricht C. Yield and constituents of greater nettle strains (Urtica

dioica L.). Zeitschrift Fur Arznei‑ and Gewurzpflanzen


Guil‑Guerrero J, Rebolloso‑Fuentes M, Torija Isasa M. Fatty acids

and carotenoids from stinging nettle (Uritca dioica L.). J Food

Compost Anal 2003;16:111‑9.

Afolayan A, Jimoh FO. Nutritional quality of some wild leafy

vegetables in South Africa. Int J Food Sci Nutr 2009;60:424‑31.

Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MN.

Role of antioxidants in prophylaxis and therapy: A pharmaceutical

perspective. J Control Release 2006;113:189‑207.

Ahangarpour A, Mohammadian M, Dianat M. Antidiabetic

effect of hydroalcohalic urtica dioica leaf extract in male rats with

fructose‑induced insulin resistance. Iran J Med Sci 2012;37:181‑6.

Farzami B, Ahmadvand D, Vardasbi S, Majin FJ, Khaghani Sh.

Induction of insulin secretion by a component of Urtica dioica

leave extract in perfused Islets of Langerhans and its in vivo effects

in normal and streptozotocin diabetic rats. J Ethnopharmacol


Al‑Wasfi RM, Jabaar Al‑Kaabee HJ, Dergham M, Hameed AF.

Studying the hypoglycemic and the antibacterial activity of various

plant extract of Urtica dioica. Al‑kufa J Biol 2012;4:232‑42.

Sidana J, Deswal G, Nain P, Arora K. Liver toxicity and

hepatoprotective herbs. Int J Pharm Sci Rev Res 2011;9:121‑61.

Daba MH, Abdel‑Rahman MS. Hepatoprotective activity of

thymoquinone in isolated rat hepatocytes. Toxicol Lett 1998;95:23‑9.

Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E.

The role of Urtica dioica and Nigella sativa in the prevention of

carbon tetrachloride‑induced hepatotoxicity in rats. Phytother

Res 2003;17:942‑6.

Kandis H, Karapolat S, Yildirim U, Saritas A, Gezer S, Memisogullari R.

Effects of Urtica dioica on hepatic ischemia‑reperfusion injury in

rats. Clin (Sao Paulo) 2010;65:1357‑61.

Daher CF, Baroody KG, Baroody GM. Effect of Urtica dioica

extract intake upon blood lipid profile in the rats. Fitoterapia


Nassiri‑Asl M, Zamansoltani F, Abbasi E, Daneshi MM,

Zangivand AA. Effects of Urtica dioica extract on lipid profile

in hypercholesterolemic rats. Zhong Xi Yi Jie He Xue Bao


Avci G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I.

Antihypercholesterolaemic and antioxidant activity assessment

of some plants used as a remedy in Turkish folk medicine.

J Ethnopharmacol 2006;107:418‑23.

Dizaye K, Alberzingi B, Sulaiman S. Renal and vascular studies of

aqueous extract of Urtica dioica in rats and rabbits. Iraq J Vet Sci


Carceres A, Giron LM, Martínez AM. Diuretic activity of plants

used for the treatment of urinary ailments in Guatemala.

J Ethnopharmacol 1987;19:233‑45.

Tita B, Faccendini P, Bello U, Martinoli L, Bolle P. Urtica dioica

Linn.: Pharmacological effect of ethanol extract. Pharmacol Res


Balzarini J, Neyts J, Schols D, Hosoya M, Van Damme E,

Pneumans W, et al. The mannose‑specific plant lectins

from Cymbidium hybrid and Epipactis helleborine and

the (N‑acetylglucosamine) n‑specific plant lectin from Urtica dioica

are potent and selective inhibitors of human immunodeficiency

virus and cytomegalovirus replication in vitro. Antiviral Res


Balzarini J, Van Laethem K, Hatse S, Froeyen M, Peumans W, Van

Damme E, et al. Carbohydrate‑binding agents cause deletions

of highly conserved glycosylation sites in HIV GP120: A new

therapeutic concept to hit the achilles heel of HIV. J Biol Chem


Uncini Manganelli RE, Zaccaro L, Tomei PE. Antiviral activity

in vitro of Urtica dioica L., Parietaria diffusa M. et K. and Sambucus

nigra L. J Ethnopharmacol 2005;98:323‑7.

Turker AU, Usta C. Biological screenings of some Turkish

medicinal plant extracts for antimicrobial and toxicity activities.

Nat Prod Res 2008;22:136‑46.

Brantner A, Grein E. Antibacterial activity of plant extracts used

externally in traditional medicine. J Ethnopharmacol 1994;44:35‑40.

Keles O, Bakirel T, Alpmar A. The antibacterial activity of some

plants used for medicinal purposes against pathogens of veterinary

importance. Folia Vet 2001;1:22‑5.

Saeed A, El‑Eraqy W, Ahmed Y. Flavonoids of Urtica urens L. and

biological evaluation. Egypt J Pharm Sci 1995;36:1‑6.

Testai L, Chericoni S, Calderone V, Nencioni G, Nieri P, Morelli I,

et al. Cardiovascular effects of Urtica dioica L. (Urticaceae)

roots extracts: In‑vitro and in‑vivo pharmacological studies.

J Ethnopharmacol 2002;81:105‑9.

Legssyer A, Ziyyat A, Mekhfi H, Bnouham M, Tahri A,

Serhrouchni M, et al. Cardiovascular effects of Urtica dioica L. in

isolated rat heart and aorta. Phytother Res 2002;16:503‑7.

Magnon M, Calderone V, Floch A, Cavero I. Influence of

depolarization on vasorelaxant potency and efficacy of Ca2+ entry

blockers, K+ channel openers, nitrate derivatives, salbutamol

and papaverine in rat aortic rings. Naunyn‑Schmiedeberg’s Arch

Pharmacol 1998;358:452‑63.

Hajhashemi V, Klooshani V. Antinociceptive and anti‑inflammatory

effects of Urtica dioica leaf extract in animal models. Avicenna J

Phytomed 2013;3:193‑200.

Toldy A, Atalay M, Stadler K, Sasvari M, Jakus J, Jung KJ, et al. The

beneficial effects of nettle supplementation and exercise on brain

lesion and memory in rat. J Nutr Biochem 2009;20:974‑81.

Johnson TA, Sohn J, Inman WD, Bjeldanes LF, Rayburn K. Lipophilic

stinging nettle extracts possess potent anti‑inflammatory activity,

are not cytotoxic and may be superior to traditional tinctures for

treating inflammatory disorders. Phytomedicine 2013;20:143‑7.

Obertreis B, Giller K, Teucher T, Behnke B, Schmitz H.

Anti‑inflammatory effect of Urtica dioica folia extract in comparison

to caffeic malic acid. Arzneimittelforschung 1996;46:52‑6.

Ozen T, Korkmaz H. Modulatory effect of Urtica dioica

L. (Urticaceae) leaf extract on biotransformation enzyme

systems, antioxidant enzymes, lactate dehydrogenase and lipid

peroxidation in mice. Phytomedicine 2003;10:405‑15.

Turel I, Oto G, Ayaz E, Yilmaz O, Mercan U. Anthelmintic activity

of Urtica dioica L. in mice naturally infected with Aspiculuris

tetraptera. J Anim Vet Adv 2008;7:1642‑4.

Nahata A, Dixit V. Ameliorative effects of stinging nettle (Urtica

dioica) on testosterone‑induced prostatic hyperplasia in rats.

Andrologia 2012;44:396‑409.

Guler ER. Investigation of chemo‑prevent properties of Urtica

dioica L., in MCF7 and MDA 231 breast cancer cell lines. New J

Med 2013;30:50‑3.

Durak I, Biri H, Devrim E, Sozen S, Avci A. Aqueous extract of

Urtica dioica makes significant inhibition on adenosine deaminase

activity in prostate tissue from patients with prostate cancer.

Cancer Biol Ther 2004;3:855‑7.

Lasheras B, Turillas P, Cenarruzabeitia E. Etude pharmacologique

preliminaire de Prunus spinosa L. Amelanchier ovalis medikus,

Juniperus communis L. et Urtica dioica L. Plant Med Phytother


Tekin M, Ozbek H, Him A. Investigation of acute toxicity,

anti‑inflammatory and analgesic effect of Urtica dioica L. Pharmacol

Online 2009;1:1210‑5.

Starkenstein E, Wasserstrom T. Pharmakologische und chemische

Untersuchungen usber die wirksamen Bestandteile der Urtica

dioica und Urtica urens. Naunyn‑Schmiedebergs. Arch Exp Pathol

Pharm 1933;27:137‑48.



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