In vitro anthelmintic activity of three medicinal plants against Haemonchus contortus

Tadesse Eguale, Mirutse Giday


The development of anthelmintic resistance and the high cost of conventional anthelmintic drugs led to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in-vitro experiments were conducted to determine the possible anthelmintic effects of crude aqueous and hydroalcoholic extracts of the leaves of Chenopodium ambrosioides, Lawsonia inermis and seeds of Jatropha curcas, on eggs and adult Haemonchus contortus. Both extracts of C. ambrosioides and J. curcas inhibited the hatching of eggs at a concentration less than or equal to 2 mg/ml, while the effect of L. inermis was not dose-dependent and did
not inhibit the hatching of eggs of H. contortus, significantly, at all tested concentrations. Based on their ED50, the two most potent extracts using egg hatch assay were the hydroalcoholic extract of C. ambrosioides (0.09 mg/ml) and the aqueous extract of J. curcas (0.1 mg/ml) in a decreasing order of potency. With regard to the effect of extracts on the survival of adult parasites, extracts from C. ambrosioides have shown a moderate effect, while J. curcas and L. inermis have shown no statistically significant effect on the survival of adult parasites at the concentrations tested, and the few mortality cases recorded were not dose-dependent (P < 0.05). The overall findings of the present study have shown that C. ambrosioides and J. curcas contain possible anthelmintic compounds
and further evaluation of these plants should be carried out.
Key words: Anthelmintic activity, Chenopodium ambrosioides, Haemonchus contortus, Jatropha curcas, Lawsonia inermis

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