Isolation, identification and purification of caffeine from Coffea arabica L. and Camellia sinensis L.: A combination antibacterial study
Abstract
Klebsiella pneumonia and Pseudomonas aeruginosa. Both compounds at a concentration of 2 mg/ml showed similar antibacterial activities against all tested bacteria, except for P. mirabilis, and the highest inhibitory effect was observed against P. aeruginosa using a modified agar diffusion method. The minimal inhibitory concentration (MIC) of caffeine was determined using a broth microdilution method in 96 multi-well microtitre plates. MIC values ranged from 62.5 to 250.0 μg/ml for the caffeine isolated from coffee and 62.5
to 500.0 μg/ml for green tea caffeine. Combination results showed additive effects against most pathogenic bacteria especially for P. aeruginosa, using both antibacterial assays.
Key words: Antibacterial activity, Coffea arabica, camellia sinensis, Caffeine
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DOI: https://doi.org/10.22377/ijgp.v3i1.56
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