In vitro cytotoxicity of extracts and fractions of Calotropis procera (Ait.) roots against human cancer cell lines

Madhulika Bhagat, Jatinder Singh Arora, Ajit Kumar Saxena


This study was designed to determine the antiproliferative activity of three extracts (alcoholic, hydro-aqueous and aqueous) and their fractions from the root part of Calotropis procera using human oral (KB) and central nervous system (SNB-78) cancer cell lines as a model system. KB and SNB-78 cells were cultured in the presence of extracts and fractions at various concentrations (10, 30 and100 μg/ml) for 48 h, and the percentage of cell viability was evaluated by the sulforhodamine-B (SRB) assay. Our result indicates that out of the three extracts of C. procera (root), alcoholic extract had shown greater potential for growth inhibition followed by hydro-aqueous extract at three different concentration of 10 μg/ml, 30 μg/ml and 100 μg/ml in a dose-dependent manner, whereas aqueous extract was found to be least active against both oral and CNS human cancer lines. On evaluation of the fractions prepared from alcoholic and hydro-aqueous extracts, it was observed that chloroform fraction from alcoholic extract was antiproliferative for oral (KB) cancer cell line and n-butanol fraction from alcoholic extract was antiproliferative for CNS cancer cell line than remaining
fractions at three different concentration of 10 μg/ml, 30 μg/ml, 100 μg/ml  in a dose-dependent manner. Thus, our result indicates that the root part of C. procera possess in vitro cytotoxicity against oral and CNS human cancer cell lines. Further investigations are required to obtain the clinically important lead molecules for the drug development.
Key words: Cytotoxicity, Calotropis procera, fractions, human cancer cell line extracts

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Yates JS, Mustian KM, Morrow GR, Gillies LJ, Padmanaban D,

Atkins JN, et al. Prevalence of complementary and alternative

medicine use in cancer patients during treatment. Support Care

Cancer 2005;13:806-11.

Cragg GM, Boyd MR, Khanna R, Kneller R, Mays TD, Mazan KD, et

al. International collaboration in drug discovery and development:

the NCI experience. Pure Appl Chem 1999;71:1619-33.

Krisharaju AV, Rao TV, Sundararaju. Assessment of bioactivity

of Indian medicinal plants using Brine shrimp (Altenaria salania)

lethality assay. Int J Appl Sci Engg 2005;2:125-34.

Kumar VL, Arya S. Medicinal uses and pharmacological properties

of Calotropis procera. In: Govil JN,ed. Recent Progress in Medicinal

Plants. Texas: Studium Press 2006;11:373-88.

Smit HF, Woerdenbag HJ, Singh RH, Meulenbeld GJ, Labadie

RP, Zwaving JH. Ayurvedic herbal drugs with possible cytostatic

activity. J Ethanopharmacol 1995;47:75-84.

Arya S, Kumar, VL. Antiinflammatory efficacy of extracts of latex

of calotropis procera against different mediators of inflammations.

Mediators inflamm 2005;14:228-32.

Chairungsrilerd N, Takeuchi K, Ohizumi Y, Ohta T, Nozoe S.

Mangostanol, a prenyl xanthone from mangostana. Phytochemistry


Bhahwal AS, Kumar A, Gupta P, Sharma M, Sethi VK, Saxena AK,

et al. Cytotoxic and apoptotic activities of novel amino analogues

of boswellic acids. Bioorg Med Chem Lett 2007;17:6411-6.

Van Quaquebeke E, Simon G, Andre A, Dewelle J, Yazidi ME,

Bruyneel F, et al. Identification of a novel cardenolide (2”-

oxovoruscharin) from Calotropis procera and the hemisynthesis of

novel derivatives displaying potent in vitro antitumor activities

and high tolerance: structure-activity relationship analysis. J Med

Chem 2005;48:849-56.

Choedon T, Mathan G, Arya S, Kumar VL, Kumar V. Anticancer

and cytotoxic properties of the Calotropis procera in a transgenic

mouse model of hepatocellular carcinoma. World J Gastroenterol


Hassan FL, Bilbis FL, Ladan MJ, Umar RA, Dangoggo SM, Saidu Y,

et al. Evaluation of antifungal activity and phytochemical analysis

of leaves, roots and stem barks extracts of Calotropis procera

(Asclepiadaceae) Pak J Biol Sci 2006;9:2624-9.

Atal CK, Setui PD. Isolation, properties and Kinetics studies of

calotropis. Planta Med 1962;10:77.



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