Productivity and health markers for large cattle

Abstract

Aim: For the first time, the Ural region reported positive results on the study of syncytial test diagnostic value at an early detection of the cattle leukemia virus carriers. Method: Study includes an intraperitoneal infection of laboratory animals (small ruminants) by the leukocyte suspension containing viral material. To perform the comparative diagnostic studies, they developed a group of animals kept in isolation, weekly studied by serological (infectious disease requisition [IDR] and enzyme-linked immunosorbent assay [ELISA]), molecular genetic (polymerase chain reaction [PCR]), cultural (ST) diagnostic research starting from the 6th day after infection. Results: Performed laboratory studies confirmed the diagnostic value of direct method identification concerning the causative agent of cattle leukemia. Thus in the earliest period (13 days) after the artificial infection of laboratory animals, they revealed a positive reaction in the syncytial method of research, which is confirmed by the controls. Positive PCR was revealed in 21 days, during the third study, and IDR and ELISA were revealed only on the 37th day (week 5). Conclusion: The developed early diagnosis of leukemia will improve the efficiency of agricultural enterprise recovery from the infection as the isolation of infected animals during the earliest period is one of the priority tasks during the implementation of health programs.