Evaluation of in vitro toxicity of Rumalaya liniment using mouse embryonic fibroblasts and human keratinocytes

Sandeep Ravi Varma, Ashok Godavarthi, Satyakumar Vidyashankar, Krishna S. Nandakumar, Pralhad Sadashiv Patki


The skin irritation potential of topical formulations is investigated prior to human exposure to identify the chemicals which might
induce adverse skin reactions. Rumalaya liniment (RL) a novel formulation using natural oils and plant extracts is used for reducing inflammations associated with musculoskeletal disorders. Preclinical studies on RL are needed prior to skin application. The aim of the study was to evaluate the possible cytotoxic effects of RL and a commercial sample (CS) on mouse embryo fibroblasts and human keratinocytes using neutral red uptake, (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and resazurin assay. The CTC50 values obtained for RL was significantly higher than that of CS, which revealed that RL is less toxic to CS. RL was less toxic (<17%) on both cell lines at 400 μg/ml and was nontoxic at further lower concentrations, whereas the toxicity of CS was above 59% even at 400 μg/ml. It was observed from the present study that by using three different assay methods and two different
cell lines, the toxicity of RL was significantly lower than that observed with CS. From the study, it could be concluded that RL could be safer to skin due to their low cytotoxicity as compared with CS.
Key words: Cell based assay, cell lines, cytotoxicity, herbal formulation, skin irritation

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DOI: http://dx.doi.org/10.22377/ijgp.v5i1.165


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