Protective effect of Amorphophallus campanulatus tuber extracts against H2O2 induced oxidative damage in human erythrocytes and leucocytes

Souravh S. Bais, Prashant Y. Mali


Background: Amorphophallus companulatus (Araceae) is a tuberous medicinal plant commonly used in Ayuvedic medicines as well as tribal medicines of India. Aim: Aim of the present study was to investigate the protective effect of A. companulatus tuber extracts against H2O2 induced oxidative damage in human erythrocytes and leucocytes. Setting and Design: The experiment was set and design as per available method in the literatures. Three measurements were performed under each set of extracts. Materials and Methods: The extracts of tuber of A. companulatus such as methanol, ethanol, acetone (70%) and hydro‑alcohol (1:1) used to assess catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH) and lipid peroxidation (LPO) levels of human erythrocytes and leucocytes. Statistical Analysis: All experimental data were statistically analysed and expressed as means ± SD
by using the one‑way analysis of variance. Results: Results of present studies revealed that, increased in the CAT, SOD, GPx and reduction of GSH and LPO levels in H2O2 group compared with control group. The extracts of tuber of A. companulatus treated groups showed effective reduction of CAT, SOD, GPx and increased the GSH and LPO levels as compared with H2O2 group on human erythrocytes and leucocytes. The methanol extract was found more effective than others. Conclusion: The present findings suggest that, the extracts of tuber of A. companulatus possess protective effect against H2O2 induced oxidative damage. Furthermore, these tuber extracts may appeared to be beneficial in preventing H2O2 oxidative human red blood cell (RBC) damage in human and can improve RBC membrane permanence. The tubers of A. companulatus are the potential source of natural antioxidants for the treatment and prevention of disease in which LPO takes place.
Key words: Amorphophallus campanulatus tuber, erythrocytes, H2O2, leucocytes, phosphate buffer solution

Full Text:



Saltman P. Oxidative stress: A radical view. Semin Hematol


Halliwell B. Introduction to free radicals in human diseases. Saudi

Med J 1991;12:13‑9.

Samuni A, Aronovitch J, Godinger D, Chevion M, Czapski G. On

the cytotoxicity of vitamin C and metal ions. A site‑specific Fenton

mechanism. Eur J Biochem 1983;137:119‑24.

Chevion M. A site‑specific mechanism for free radical induced

biological damage: The essential role of redox‑active transition

metals. Free Radic Biol Med 1988;5:27‑37.

Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants,

and the degenerative diseases of aging. Proc Natl Acad Sci U S A


Anderson D, Phillips BJ. Comparative in vitro and in vivo effects

of antioxidants. Food Chem Toxicol 1999;37:1015‑25.

Tavazzi B, Di Pierro D, Amorini AM, Fazzina G, Tuttobene M,

Giardina B, et al. Energy metabolism and lipid peroxidation of

human erythrocytes as a function of increased oxidative stress.

Eur J Biochem 2000;267:684‑9.

Rice‑Evans C, Burdon R. Free radical‑lipid interactions and their

pathological consequences. Prog Lipid Res 1993;32:71‑110.

Knight JA. Reactive oxygen species and the neurodegenerative

disorders. Annu Clini Laborat Sci 1997; 27:11‑25.

Briviba K, Sies H. Non enzymatic antioxidant defense systems.

In: Frie B, editor. Natural Antioxidants in Human Health and

Diseases. Canada: Academic Press; 1994. p. 119‑21.

Barnham KJ, Masters CL, Bush AI. Neurodegenerative diseases

and oxidative stress. Nat Rev Drug Discov 2004;3:205‑14.

Pardo‑Andreu GL, Sánchez‑Baldoquín C, Avila‑González R,

Delgado R, Naal Z, Curti C. Fe (III) improves antioxidant and

cytoprotecting activities of mangiferin. Eur J Pharmacol 2006;547:31‑6.

Sibel K, Canan K. The protective effects of Achillea L. species

native in turkey against H2O2‑induced oxidative damage in human

erythrocytes and leucocytes. J Ethnopharm 2005;102:221‑7.

Tseng TH, Wang CJ, Kao ES, Chu HY. Hibiscus protocatechuic

acid protects against oxidative damage induced by

tert‑butylhydroperoxide in rat primary hepatocytes. Chem Biol

Interact 1996;101:137‑48.

Duh P, Yen G. Antioxidant activity of three herbal water extracts.

Food Chemist 1997;60:639‑45.

Kim BJ, Kim JH, Kim HP, Heo MY. Biological screening of 100

plant extracts for cosmetic use (II): Anti‑oxidative activity and

free radical scavenging activity. Int J Cosmet Sci 1997;19:299‑307.

Asai A, Nakagawa K, Miyazawa T. Antioxidative effects of

turmeric, rosemary and capsicum extracts on membrane

phospholipid peroxidation and liver lipid metabolism in mice.

Biosci Biotechnol Biochem 1999;63:2118‑22.

Nadkarni KM. Indian Materia Medica, 3rd ed., Vol. 1. Mumbai:

Popular Prakashan; 2000. p. 94‑5.

Hedrick Nair RV. Indian Medicinal Plants 1, 2nd ed. Andra Pradesh,

India: Orient Longman; 1993. p. 118‑22.

Hedrick UP. Sturtevant›s Edible Plants of the World. New York:

Dover Publications; 1972.

Singh A. Wadhwa Neeraj. Osmotic dehydration of Amorphophallus

paeoniifolius slices and it’s phyto‑chemical investigation. Int J

Pharm Life Sci 2012;3:1797‑801.

Bhattacharya S. Chironjib Banoushadi, 1st ed., Vol. 2. Calcutta:

Anand Publishing Ltd.; 1990. p. 63‑9.

Ghani A. Medicinal Plants of Bangladesh. Dhaka, Bangladesh:

Asiatic society of Bangladesh; 1998. p. 77‑8.

Kirtikar KR, Basu BD. Indian Medicinal Plants, 2nd ed., Vol. 4. India:

Dehradun Publisher Ltd.; 1994. p. 2609‑10.

Sharstry RA, Biradar SM, Mahadevan KM, Habbu PV. Isolation

and characterization of secondary metabolite from Amorphophallus

paeoniifolius for hepatoprotective activity. Res J Pharma Bio Chem

Sci 2010;1:429‑37.

Hurkadale PJ, Shelar PA, Palled SG, Mandavkar YD, Khedkar AS.

Hepatoprotective activity of Amorphophallus paeoniifolius tubers

against paracetamol‑induced liver damage in rats. Asian Pac J

Trop Biomed 2012:Supplement 1:S238‑42.

Jain S, Dixit VK, Malviya N, Ambawatia V. Antioxidant and

hepatoprotective activity of ethanolic and aqueous extracts

of Amorphophallus campanulatus Roxb. tubers. Acta Pol Pharm


Singh SK, Rajasekar N, Armstrong Vinod Raj N, Paramaguru R.

Hepatoprotective and antioxidant effects of Amorphophallus

campanulatus against acetaminopheninduced hepatotoxicity in

rats. Int J Pharm Pharmaceut Sci 2011;3:202‑5.

Bais S, Singh K, Bigoniya P, Rana AC. The in‑vitro antioxidant

and free radical scavenging Activities of suran (Amorphophallus

campanulatus (Araceae)) tubers extracts. Int J Pharm Life Sci


Sahu KG, Khadabadi SS, Bhide SS. Evaluation of in‑vitro

antioxidant activity of Amorphophallus campanulatus (Roxb.) Ex

blume Decne. Int J Chem Sci 2009;7:1553‑62.

Basu S, Sen A, Das M, Nath P, Datta G. Phytochemical evaluation

and in‑vitro study of antioxidant potency of Amorphophallus

campanulatus, Alocasia indica and Colocasia esculenta: a comparative

analysis. Int J Pharm Bio Sci 2012;3:170‑80.

Krishna KR, Kumari SK, Vinodha M, Sebastin A, Gomathi R.

In‑vitro free radical scavenging activity of Amorphophallus

campanulatus an endemic aroid of Western Ghats, South India. Int

Res J Pharm 2012;3:133‑7.

Ramesh CK, Raghu KL, Jamuna KS, Govinden SJ,

Ranghoo‑Sanmukhiya VM, Vijay Avin BR. Comparative evaluation

of antioxidant property in methanol extracts of some common

vegetables of India. Ann Biol Res 2011;2:86‑94.

Ansil PN, Nitha A, Prabha SP, Wills PJ, Jazaira V, Latha MS.

Protective effect of Amorphophallus campanulatus (Roxb.) Blume.

tuber against thioacetamide induced oxidative stress in rats. Asian

Pac J Trop Med 2011;4:870‑7.

Jagatheesh K, Arumugam V, Elangovan N, PavanKumar P.

Evaluation of the anti‑tumor and antioxidant activity of

Amorphophallus Paeonifolius on DMBA induced mammary

carcinoma. Int J Chem Pharm Sci 2010;1:40‑50.

Dey YN, De S, Ghosh AK. Evaluation of analgesic activity of

methanolic extract of Amorphophallus paeoniifolius tuber by tail flick

and acetic acid‑induced writhing response method. Int J Pharm

Bio Sci 2010;1:662‑8.

Shilpi JA, Ray PK, Sarder MM, Uddin SJ. Analgesic activity of

Amorphophallus campanulatus tuber. 2005;76:367‑9.

Patel K, Gupta M, Page A. Evaluation of analgesic and

anti‑inflammatory activity of methanolic extract of Amorphophallus

campanulatus tuber by carrageenan induced paw oedema and hot

plate method. J Adv Pharm Healthcare Res 2011;1:31‑6.

Angayarkanni J, Ramkumar KM, Poornima T, Priyadarshini U.

Cytotoxic activity of Amorphophallus campanulatus tuber extracts

In‑vitro. American‑Eurasian J Agri Environ Sci 2007;4:395‑8.

Khan A, Rahman M, Islam MS. Antibacterial, antifungal and

cytotoxic activities of 3,5‑diacetyltambulin isolated from

Amorphophallus campanulatus Blume ex. Decne. Daru 2008;16:239‑44.

Khan A, Rahman M, Islam MS. Antibacterial, antifungal and

cytotoxic activities of amblyone isolated from Amorphophallus

campanulatus. Indian J Pharmacol 2008;40:41‑4.

Khan A, Rahman M, Islam MS. Antibacterial, antifungal and

cytotoxic activities of salviasperanol isolated from Amorphophallus

campanulatus. Pharma Bio 2009;47:1187‑91.

Khan A, Rahman M, Islam S. Antibacterial, antifungal and cytotoxic

activities of tuberous roots of Amorphophallus campanulatus. Turk J

Biol 2007;31:167‑72.

Dey YN, Ghosh AK. Evaluation of anthelmintic activity of the

methanolic extract of amorphophallus paeoniifolius tuber. Int J Pharm

Sci Res 2010;1:117‑21.

Ramalingam R, Hima Bindu K, Bindu Madhavi B, Ravinder Nath A,

David Banji D. Phyto chemical and anthelmintic evaluation of corm

of Amorphophallus campanulatus. Int J Pharma Bio Sci 2010;1:1‑9.

De S, Dey YN, Ghosh AK. Anti‑inflammatory activity of methanolic

extract of Amorphophallus paeoniifolius and its possible mechanism.

Int J Pharma Bio Sci 2010;1:1‑8.

Ansil PN, Prabha SP, Nitha A, Wills PJ, Jazaira V, Latha MS. Curative

effect of Amorphophallus campanulatus (Roxb.) Blume. tuber methanolic

extract against thioacetamide induced oxidative stress in experimental

rats. Asian Pac J Trop Biomed 2012: Supplement 1S83‑9.

Nataraj HN, Murthy RNL, Setty RS. In‑vitro evaluation of

gastro‑protective of suran‑A possible explanation through HPLC

analysis. Int Res J Pharm 2011;1:103‑6.

Nataraj HN, Murthy RNL, Setty RS. Evaluation of gastroprotective

ability of Amorphophallus paeoniifolius corms against indomethacin

induced gastric ulcers. RGHUS J Pharm Sci 2012;1:67‑73.

Purwal L, Shrivastava V, Jain UK. Studies on antidiarrhoeal activity

of leaves of Amorphophallus paeoniifolius in experimental animals.

Int J Pharm Sci Res 2011;2:468‑71.

Tripathi1 AS, Chitra V, Sheikh NW, Mohale DS, Dewani AP.

Immunomodulatory activity of the methanol extract of

Amorphophallus campanulatus (Araceae) tuber. Trop J Pharma Res


Das SS, Sen M, Dey YN, De S, Ghosh AK. Effects of petroleum ether

extract of Amorphophallus paeoniifolius tuber on central nervous

system in mice. Indian J Pharm Sci 2009;71:651‑5.

Dey YN, De S, Ghosh AK, Gaidhani S, Kumari S, Jamal M.

Synergistic depressant activity of Amorphophallus paeoniifolius in

Swiss albino mice. J Pharmacol Pharmacother 2011;2:121‑3.

De S, Dey YN, Gaidhani S, Ota S. Effect of petroleum ether extract

of Amorphophallus paeoniifolius tuber on experimentally induced

convulsions in mice. Int J Nutir Pharmacol Neuro Disea 2012;2:132‑4.

Orhan H, Dogruer DS, Cakır B, Sahin G, Sahin MF. The in‑vitro

effects of non‑steroidal antiinflammatory compounds on

antioxidant systems of human erytrocytes. Exper Toxi Path


Cutts JH. Cell Separation, Methods in Hematology. New York:

Academic Press; 1970. p. 43.

Mikulíková D, Trnavský K. Effect of levamisole on lysosomal

enzyme release from polymorphonuclear leukocytes and

intracellular levels of cAMP and cGMP after phagocytosis of

monosodium urate crystals. Agents Actions 1980;10:374‑7.

Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121‑6.

Sedlak J, Lindsay RH. Estimation of total, protein‑bound, and

nonprotein sulfhydryl groups in tissue with Ellman’s reagent.

Anal Biochem 1968;25:192‑205.

Satoh K. Serum lipid peroxide in cerebrovascular disorders

determined by a new colorimetric method. Clin Chim Acta


Yagi K. Assay for blood plasma or serum. Methods Enzymol


Bauer JD, Ackermann PG, Toro G. Clinical Laboratory Methods.

London: C.V. Mosby Co.; 1974. p. 97.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement

with the Folin phenol reagent. J Biol Chem 1951;193:265‑75.

Bukowska B. Effects of 2,4‑D and its metabolite 2,4‑dichlorophenol

on antioxidant enzymes and level of glutathione in human

erythrocytes. Comp Biochem Physiol C Toxicol Pharmacol


Stanner SA, Hughes J, Kelly CN, Buttriss J. A review of the

epidemiological evidence for the antioxidant hypothesis. Public

Health Nutr 2004;7:407‑22.

Briviba K, Sies H. Non enzymatic antioxidant defense systems.

In: Frie B, editor. Natural Antioxidants in Human Health and

Diseases. San Diego: Academic press Inc.; 1994. p. 119‑21.

Ray G, Husain SA. Oxidants, antioxidants and carcinogenesis.

Indian J Exp Biol 2002;40:1213‑32.

Tsuda H. Chromosomal aberrations induced by hydrogen peroxide

in cultured mammalian cells. Japanese J Genet 1981;58:1‑8.

Konyalioglu S, Karamenders C. Screening of total flavonoid,

phenol contents and antioxidant capacities of Achillea L. species

growing in Turkey. Acta Pharma Turcia 2004;46:163‑70.

Ault JG, Lawrence DA. Glutathione distribution in normal and

oxidatively stressed cells. Exp Cell Res 2003;285:9‑14.



  • There are currently no refbacks.