Comparative estimation of β‑sitosterol in roots, leaves and flowers of Clerodendrum infortunatum L.

Suman Verma, Rajiv Gupta


Background: Clerodendrum infortunatum L. (family Verbenaceae) commonly known as Bhant, plays a significant role in Indian System of Medicine, i.e., Ayurveda, due to its medicinal properties. The plant is used in the treatment of bronchitis, asthma, fever, diseases of the blood, inflammation, burning sensation and epilepsy. Aim: High performance thin layer chromatography (HPTLC) is an important tool used qualitatively as well as quantitatively for the purity and identity determinations of crude drugs and one of the major recent advances in the area of standardization, and also to keep a check on adulteration. In the present study, HPTLC has been
developed for detection and quantification of β‑sitosterol in various parts of C. infortunatum L. Materials and Methods: Increasing serial dilutions of reference standard β‑sitosterol (200‑1000 µg/ml) were scanned at 273 nm to detect and quantify the concentrations of β‑sitosterol in test samples. Results and Discussion: The estimated amount of β‑sitosterol on per gram basis of crude powder was found to be 7.96 mg/g, 4.23 mg/g and1.92 mg/g in roots, leaves and flowers, respectively. Conclusion: The method provided a rapid and easy approach for detection and the quantitation of the bio‑marker β‑sitosterol. In the present study, we established the HPTLC profile for the vegetative and reproductive parts of C.infortunatum L. to detect and quantify the β‑sitosterol.
Key words: Bhant, Clerodendrum infortunatum L., high performance thin layer chromatography, β‑sitosterol

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