Comparative study of in vitro antidiabetic activity with ethyl acetate, aqueous extracts containing seeds of Trigonella foenum-graecum

Abstract

The aims of this study were to compare the in vitro antidiabetic activity with ethyl acetate and aqueous
extract containing seeds of Trigonella foenum-graecum. Materials and Methods: The seeds are dried, powdered,
and extracted with the solvents among this ethyl acetate and aqueous extract of fenugreek seeds which were
chosen. Phytochemical screening shows the presence of flavonoids, phenol, glycosides, and steroids. In vitro
antidiabetic potential was prepared by an aggregate of 500 μL of test samples and standard drug metformin
(100–1000 μg/mL) was added to 500 μL of 0.20 mM, and phosphate buffer (pH6.9) containing α-amylase
(0.5 mg/mL) and was incubated at 25°C for 10 min. After these, 500 μL of a 0.02 M sodium phosphate buffer
(pH6.9) was added to each tube, were incubated at 25°C for 10 min. The response was stopped with 1.0 mL of
3, 5 dinitrosalicylic acid color reagent. The test tubes were also incubated in a scorching water bath for 5 min,
cooled to room temperature. The response recorded by adding 10 mL of distilled water and absorbance was
measured at 540 nm. Results and Discussion: There was an increased inhibition by T. foenum-graecum seed
extracts in a dose-dependent manner. As the concentration of the inhibitor increased, the amylase enzyme activity
was decreased. It was concluded that the ethyl acetate extract showed better inhibitory activity compared with
the standard and aqueous extract of T. foenum-graecum seeds. Inhibition was found to be dose-dependent by
standard drug metformin which is a commercially available alpha-amylase inhibitor for the treatment of diabetes.
Conclusion: The present study entitled the “Relative study of in vitro antidiabetic activity with ethyl acetate,
waterless excerpt containing seeds of T. foenum-graecum†focuses on a factory that is generally available
throughout India and traditionally used in the treatment of diabetics.