Thearubigins rich black tea fraction reveals strong antioxidant activity

Sangeeta Kumari Sinha, Saroj S. Ghaskadbi

Abstract


Background: Black tea contains two major polyphenols namely Theaflavins (TFs) and Thearubigins (TRs). Several reports are available on antioxidant properties of TFs. However, very little information is known about TRs. Aim: To investigate in vitro antioxidant properties of not well‑known TR rich fractions in comparison with TF rich fraction. Materials and Methods: TR and TF rich fractions were checked for in vitro antioxidant activities by using standard biochemical radical scavenging assays and pulse radiolysis. These fractions were also used to evaluate the protection conferred against induced oxidative damage to biomolecules (lipids, proteins and DNA) in rat liver mitochondria. Statistical Analysis: The one‑way analysis of variance test associated with the Tukey’s test was used to determine the statistical significance of the differences among experimental groups. All the statistical analyses were done using SPSS 19.0 software. Results and Conclusions: Results from this study suggest that like TF rich fraction, TR rich fraction has
good antioxidant properties, which correlate to the total phenolics and flavonoids content and provide significant protection against
oxidative damage to biomolecules (lipids, proteins and DNA) in rat liver mitochondria. This is the first report about the in vitro
antioxidant activity of TRs rich fractions from black tea.
Key words: Antioxidant, black tea, oxidative damage, theaflavins, thearubigins

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References


Balentine DA, Wiseman SA, Bouwens LC. The chemistry of tea

flavonoids. Crit Rev Food Sci Nutr 1997;37:693‑704.

Blot WJ, McLaughlin JK, Chow WH. Cancer rates among drinkers

of black tea. Crit Rev Food Sci Nutr 1997;37:739‑60.

Cabrera C, Artacho R, Gimenez R. Beneficical effects of green tea:

A review. J Am Coll Nutr 2006;25:79‑99.

Jhoo JW, Lo CY, Li S, Sang S, Ang CY, Heinze TM, et al. Stability

of black tea polyphenol of, theaflavin, and identification

theanaphthoquinone as its major radical reaction product. J Agric

Food Chem 2005;53:6146‑50.

Sun S, Pan S, Ling C, Miao A, Pang S, Lai Z, et al. Free radical

scavenging abilities in vitro and antioxidant activities in vivo of

black tea and its main polyphenols. J Med Plants Res 2012;6:114‑21.

Ngure FM, Wanyoko JK, Mahungu SM, Shitandi AA. Catechins

depletion patterns in relation to theaflavin and thearubigins

formation. Food Chem 2009;115:8‑14.

Miller NJ, Castelluccio C, Tijburg L, Rice‑Evans C. The antioxidant

properties of theaflavins and their gallateesters‑radicalscavengers

or metal chelators. FEBS Lett 1996;392:40‑4.

Yang Z, Jie G, Dong F, Xu Y, Watanabe N, Tu Y. Radical‑scavenging

abilities and antioxidant properties of theaflavins and their

gallate esters in H2O2‑mediated oxidative damage system in the

HPF‑1 cells. Toxicol In Vitro 2008;22:1250‑6.

Rice‑Evans CA, Miller NJ, Paganga G. Antioxidant properties of

phenolic compounds. Trends Plant Sci 1997;2:152‑9.

Ozawa T, Kataoka M, Morikawa K, Negishi O. Elucidation of

the partial structure of polymeric thearubigins from black tea by

chemical degradation. Biosci Biotech Biochem 1996;60:2023‑7.

Halder B, Bhattacharya U, Mukhopadyay S, Giri AK. Inhibition

of benzo[a] pyrene induced mutagenicity and genotoxicity by

black tea polyphenols theaflavins and thearubigins in multiple

test systems. Food Chem Toxicol 2006;43:591‑7.

Halder B, Bhattacharya U, Mukhopadyay S, Giri, AK. Molecular

mechanism of black tea polyphenols induced apoptosis in

human skin cancer cells: Involvement of Bax translocation

and mitochondria mediated death cascade. Carcinogenesis

;29:129‑38.

Shon MY, Parkm SK, Nam SH. Antioxidant activity of theaflavins

and thearubigins separated form Korean microbially fermented

tea. J Food Sci Nutr 2007;12:7‑10.

Aquino R, Morelli S, Lauro MR, Abdo S, Saija A, Tomaino A.

Phenolic constituents and antioxidant activity of an extract of

Anthuriumversicolor leaves. J Nat Prod 2001;64:1019‑23.

Pulido R, Bravo L, Saura‑Calixto F. Antioxidant activity of

dietary polyphenols as determined by a modified ferric reducing/

antioxidant power assay. J Agric Food Chem 2000;48:3396‑402.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity

and inhibition of intercellular communication by antioxidant

catechins isolated from Chinese green tea. Carcinogenesis

;10:1003‑8.

Nishikimi M, Appaji N, Yogi K. The occurrence of superoxide anion

in the reaction of reduced phenazine methosulfate and molecular

oxygen. Biochem Biophys Res Commun 1972;46:849‑54.

Sreejayan N, Rao MN, Priyadarsini KI, Devasagayam TP. Inhibition of radiation induced lipid peroxidation by curcumin. Int J Pharm

;151:127‑30.

Alzoreky N, Nakahara K. Antioxidant activity of some edible

Yemeni plants evaluated by ferrylmyoglobin/ABTS•+ assay. Food

Sci Technol Res 2000;7:141‑4.

Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method:

A simple “test tube” assay for determination of rate constants for

reactions of hydroxyl radicals. Anal Biochem 1987;165:215‐9.

Dixit P, Ghaskadbi S, Mohan H, Devasagayam TP. Antioxidant

properties of germinated fenugreek seeds. Phytother Res

;19:977‑83.

Naik GH, Priyadarsini KI, Mohan H. Free radical scavenging

reactions and phytochemical analysis of triphala, an ayurvedic

formulation. Curr Sci 2006;90:1100‑5.

Londhe JS, Devasagayam TP, Foo LY, Ghaskadbi SS. Radioprotective

properties of polyphenols from phyllanthus amarus Linn. J Radiat

Res 2009;50:303‑9.

Singleton VL, Orthofer R, Lamuela‐Raventos RM. Analysis of total

phenols and other oxidation substrates and antioxidants by means

of Folin‐Ciocalteaure agent. Method Enzymol 1999;299:152‐78.

Zhinshen J, Mengcheng T, Jianming W. Research on antioxidant

activity of flavonoids from natural materials. Food Chem

;64:555‐9.

Katyare SS, Rajan RR. Enhanced oxidative phosphorylation in rat

liver mitochondria following prolonged in vivo treatment with

Imipramine. Br J Pharmacol 1988;95:914‑22.

Lowry ΟΗ, Rosebrough ΝJ, Farr AL, Randall RJ. Protein measurement

with Folin‑phenol reagent. J Biol Chem 1951;193:265‑75.

Devasagayam TP. Lipid peroxidation in rat uterus. Biochem

Biophys Acta 1986;876:507‑14.

Nourooz‑Zadeh J, Tajaddini‑Sarmadi J, Ling KL, Wolff SP.

Low‑density lipoprotein is the major carrier of lipid hydroperoxides

in plasma. Relevance to determination of total plasma lipid

hydroperoxide concentrations. Biochem J 1996;313:781‑6.

Palamanda JR, Kehrer JP. Inhibition of protein carbonyl formation

and lipid peroxidation by glutathione in rat liver microsomes. Arch

Biochem Biophys 1992;293:103‑9.

Ellmann GL. Tissue sulfhydryl groups. Arch Biochem Biophys

;82:70‑7.

Modak MA, Parab PB, Ghaskadbi SS. Pancreatic islets are very

poor in rectifying oxidative DNA damage. Pancreas 2009;38:23‑9.

Zhao S, Weng YC, Yuan SS, Lin YT, Hsu HC, Lin SC, et al.

Functional link between ataxia‑telangiectasia and Nijmegen

breakage syndrome gene products. Nature 2000;405:473‑7.

Roy S, Qiao T, Wolff C, Ingham PW. Hedgehog signaling pathway

is essential for pancreas specification in the zebrafish embryo. Curr

Biol 2001;11:1358‑63.

Wang T, Liu YY, Wang X, Yang N, Zhu HB, Zuo PP. Protective effects

of octacosanol on 6‑hydroxydopamine‑induced Parkinsonism in

rats via regulation of ProNGF and NGF signaling. Acta Pharmacol

Sin 2010;31:765‑74.

Adhikari BN, Wall DH, Adams BJ. Desiccation survival in

an Antarctic nematode: Molecular analysis using expressed

sequenced tags. BMC Genomics 2009;10:69.

Hara A. Studies on female‑specific serum protein (vitellogenin) and

egg yolk proteins in teleosts: Immunochemical, physicochemical

and structural studies. Memoirs of the Faculty of Fisheries,

Kagoshima University 1987;34:1‑59.

Halder B, Pramanick M, Mukhopadhyay S, Giri AK. Anticlastogenic

effects of black tea polyphenols theaflavins and thearubigins in

human lymphocytes in vitro. Toxicol In Vitro 2006;20:608‑13.

Magalhães LM, Segundo MA, Reis S, Lima JL. Methodological

aspects about in vitro evaluation of antioxidant properties. Anal

Chim Acta 2008;613:1‑19.

Shalaby EA, Shanab SM, Vikramjit S. Salt stress enhancement of

antioxidant and antiviral efficiency of Spirulina platensis. J Med

Plants Res 2010;4:2622‑32.

Shiraki M, Hara Y, Osawa T, Kumon H, Nakayama T, Kawakishi S.

Antioxidative and antimutagenic effects of theaflavins from black

tea. Mutat Res 1994;323:29‑34.

Yoshino K, Tomita I, Sano M, Oguni I, Hara Y, Nakano M. Effects of

long term dietary supplement of tea polyphenols on lipid peroxide

levels in rats. Age 1994;17:79‑85.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals,

metals and antioxidants in oxidative stress‑induced cancer. Chem

Biol Interact 2006;160:1‑40.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J.

Free radical and antioxidant in normal physiological functions

and human diseases. Int J Biochem Cell Biol 2007;39:44‑84.

Xu BJ, Chang SK. A comparative study on phenolic profiles and

antioxidant activities of legumes as affected by extraction solvents.

J Food Sci 2007;72:S159‑66.

Haslam E. Thoughts on thearubigins. Phytochemistry 2003;64:61‑73.

Yao LH, Jiang YM, Caffin N, Arcy BD, Datta N, Liu X, et al. Phenolic

compounds in tea from Australian super markets. Food Chem

;96:614‑20.

Kuhnert N. Unraveling the structure of the black tea thearubigins.

Arch Biochem Biophys 2010;501:37‑51.




DOI: http://dx.doi.org/10.22377/ijgp.v7i4.345

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