Gas chromatographyâˆ’Mass spectrometry analysis and antibacterial activity of essential oil from aerial parts and roots of Anisomeles indica Linn

Yogesh V. Ushir; Anil U. Tatiya; Sanjay J. Surana; Ulhas K. Patil

doi:10.22377/ijgp.v4i2.427

Gas chromatographyâˆ’Mass spectrometry analysis and antibacterial activity of essential oil from aerial parts and roots of Anisomeles indica Linn

Yogesh V. Ushir, Anil U. Tatiya, Sanjay J. Surana, Ulhas K. Patil

Abstract

The essential oil from the aerial part and roots of Anisomeles indica Linn. from the Toranmal forest, Maharashtra (India), was obtained by hydrodistillation after drying and grounding of the herbal material. A total of 27 components were resolved in the essential oil by GC-MS analysis, while 18 compounds were identified comparing the recorded mass spectra with the Nist 98/Nbs 75 K GC-MS library of mass spectra. The microbiological activity of the isolated essential oils was investigated as well. It was found that the essential oil shows microbiological activity on Escherichia coli, Pseudomonas aeruginosa, Bacillus pumilus and Staphylococcus aureus with a range of minimum inhibitory concentration values extended from 31.25 to 250 Î¼g/ml. The percentage inhibition values calculated also support the minimum inhibitory concentration findings. The MBCs also performed for the same pathogens. Limonene, pinene,
isobornyl acetate and eugenol can be considered as the main antimicrobial constituents in the essential oils of plants.
Key words: Anisomeles indica Linn, essential oil, microbiological activity

Full Text:

Untitled

References

Kirtikar KR, Basu BD. Indian Medicinal Plants Vol. 3. 6th ed.

Dehradun, India: International Book Distributors; 1999. p. 2212-4.

Anonymous, The Wealth of India, Raw Materials, Vol 6. New Delhi:

Publication and Information Directorate, CSIR; 2003. p. 295-6.

Dharmasiri M, Thabrew M, Ratnasooriya W. Anti-inflammatory

effects of Anisomeles indica. Phytomed 2000;7:97.

Dharmasiri M, Thabrew M, Ratnasooriya W. Water extract of

leaves and stems of Anisomeles indica possesses analgesic and

antihyperalgesic activities in rats. Pharmaceutical Bio 2003;41:

-44.

Alam M, Quader M, Rashid M. HIV-inhibitory diterpenoid from

Anisomeles indica. Fito 2000;71:574-6.

Anonymous, Second Supplement to Indian medicinal plants with

active principles, part- I, A-K, 1965-1981. p. 70.

Quality Control methods for medicinal plant material by WHO,

Geneva. Delhi: A.I.T.B.S. Publishers and Distributors; 2002.

p. 34-7, 64-73.

Guo F, Liang Y, Xu C, Li X, Huang L. Analysis of the volatile

chemical constituents in Artemisia capillaries herba by GC-MS

and correlative chemometric resolution methods. J Pharma Biomed

Ana 2004;35:469-78.

Howes M, Simmonds S, Kite G. Evaluation of the quality of

sandalwood oils by gas chromatography â€“ mass spectrometry. J

Chromatogr A 2004;1028:307-12.

Saxena VK, Sharma RN. Antimicrobial activity of the essential oil

of Lantana aculeata. Fito 1999;70:67-70.

Tabanca N, Demirci B, Kirimer N, Baser K, Bedir E, Khan I, et al.

Gas chromatographic-mass spectrometric analysis of essential oils

from Pimpinella aurea, Pimpinella corymbosa, Pimpinella peregrina and

Pimpinella puberula gathered from Eastern and Southern Turkey. J

Chromatogr A 2005;1097:192-8.

Basic Laboratory Procedures in Clinical Bacteriology, World Health

Organisation. Geneva: Jaypee Brothers; 1991. p. 84.

Applied microbiology analysis. In: Rajvaidya N, Markandey DK,

editors. New Delhi: A.P.H. Publishing Corporation; 2004. Vol. 5,

p. 315-7.

Trease GE, Evans MC. Textbook of Pharmacognosy. 15th ed.

London: Balliere, Tindall; 1983. p. 383, 519-21.

Tyler V, Brady L, Robbers J. Pharmacognosy. 8th ed. India: K. M.

Varghese Company; 1977. p. 141.

Inouye S, Takizawa T, Yamaguchi H. Antibacterial activity of

essential oils and their major constituents against respiratory

tract pathogens by gaseous contact. J Antimicrob Chemother

;47:565-73.

Georgea J, Ravishankara GA, Keshavab N, Udayasankarc K,

Antibacterial activity of supercritical extract from Decalepis

hamiltonii roots. Fito 1999;70:172-4.

DOI: https://doi.org/10.22377/ijgp.v4i2.427

Refbacks

There are currently no refbacks.

Username
Password
Remember me