Analytical Standardization Assessment ofDushivishari Agad

Abstract

Dushivishari Agada (DVA) is an Ayurvedic herbo-mineral formulation having a vital role in
inflammatory disorders of skin due to its anti-inflammatory, antioxidant, and immunomodulatory properties. DVA
comprises Pippali (Piper longum Linn.), Dhyamaka (Cymbopogon martinii (Roxb.) Wats.), Jatamansi (Nardostachys
jatamamsi (DC.), Musta (Cyporusrotundus) Lodhra (Symplococus racemosa Roxb.), Ela (Elettaria cardamomum
Maton.), Suvarchika (Salt petre), Kutannatum (Oroxylum indicum (L.) Benth. ex Kurz), Natam (Valeriana wallichii),
Kushta (Saussurea lappa DC.), Yashtimadhu (Glycyrrhiza glabra L.), Chandana (Santalum album L.), and Gairika
(Red Ochre Fe2O3) which is used to combat residual toxicity. Standardization of self-prepared herbo-mineral
formulation of DVA is important for purity, safety, and efficacy of the product. Objectives: The present study
aimed to standardize DVA and its contents, employing a standard testing protocol for AYUSH drugs. Materials
and Methods: DVA was prepared according to Ashtang Hridaya and the Analytical profile was evaluated as per
WHO guidelines, Ayurvedic Pharmacopoeia of India and Indian Pharmacopoeia by Organoleptic, Physicochemical,
Phytochemical, Microbiological and Chromatographical parameters. Results: Organoleptic and Physicochemical
parameters have proved the color, odor, taste, appearance, purity, and strength of DVA. Phytochemical test proved
the presence of alkaloids, glycosides, flavonoids, amino acids, steroids, saponines and tannins. High-performance
thin-layer chromatography proved the presence of 8 phytoconstituents in DVA. Conclusion: The analytical
assessment revalidates the standardization of DVA in the given standard conditions of the study