Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill. leaves

M. Mujeeb; V. Aeri; P. Bagri; S. A. Khan

doi:10.22377/ijgp.v3i2.68

Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill. leaves

M. Mujeeb, V. Aeri, P. Bagri, S. A. Khan

Abstract

The methanolic extract of Tylophora indica leaves was screened for hepatoprotective activity in carbon tetrachloride induced
hepatotoxicity in albino rats. The degree of protection was measured by estimating biochemical parameters like Serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, total protein and level of serum bilirubin (both total and direct). Hepatoprotective activity of methanolic extract at a dose of 200 mg/kg and 300 mg/kg body weight, i.p., was compared with Silymarin (25 mg/kg, i.p.) treated animals. Tylophora indica leaves (200 and 300 mg/kg) exhibited significant reduction in serum hepatic enzymes when compared to rats treated with carbon tetrachloride alone. Furthermore, histopathological studies were also
done to support the study.
Key words: Carbon tetrachloride, hepatoprotective activity, Silymarin, Tylophora indica

Full Text:

PDF

References

Anonymous. The Wealth of India. National Institute of Science and

Communication,CSIR, New Delhi, India, 1978. p398 - 399.

Phillipson JD, Tezcan I, Hylands PJ. Alkaloids of Tylophora species

from Sri Lanka. Planta Med 1974;25:301-9.

Gopalkrishan C, Shakaranarayana D, Kameswaran L.

Pharmacological investigation of Tylophorine, a major alkaloid

of Tylophora indica. Indian J Med Res 1979;69:513-20.

Govindchari TR, Viswanathan N, Radhakrishnan J. Tylophora

alkaloids. J Ind Chem Soc 1973; Vol C; 1-9.

Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal

Plants. Vol. 1. Luknow: CSIR New Delhi and CDRI;1995. p. 423-4.

Chopra RN, Nayar SC, Chopra IC. Glossary of Indian medicinal

plants. New Delhi: CSIR publication; 1956. p. 250-1.

Ganguli T, Sainis KB. Inhibition of cellular immune responses

by Tylophora indica in experimental models. Phytomedicine

;895:348-55.

Gangluli T, Khar A. Induction of apoptosis in a human

erythroleukemic cell lines K- 562 by Tylophora alkaloid

involves release of cytochrome C and activation of Caspase 3.

Phytomedicine 2002;9:288-95.

Gopalkrishnan C, Shakaranarayana D, Nazimudin SK and

Kameswaran L. Effect of Tylophorine, a major alkaloid of

Tylophora indica on immunopathological and inflammatory

reactions. Ind J Med Res 1980;71:940-8.

Bhutani KK, Sharma GL, Ali M. Plant based antiamoebic drugs

part I: Anti amoebic activity of Phenanthroindolizidine alkaloids;

common structural determinants of activity with emetine. Planta

med 1987;53(6):532-6.

Rietman S, Frankel S. A colorimetric method for the determination

of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin. Pathol 1957;28:56-63.

Malloy HT, Evelyn KA.The determination of bilirubin with the

photometric colorimeter. J Biol Chem 1937;119:481-90.

Luna LG. Manual of histology. Staining methods of Armed Force

Institute of Pathology. 3rd ed. Mc Graw Hill Book Co. Ltd: 2002.

Woolson RF. Statistical methods for the analysis of biomedical

data. New York: John Wiley and Sons Inc; 1987.

Johnson DE, Kroening C. Mechanism of early Carbon tetrachloride

toxicity in cultured rat hepatocytes. Pharmacol Toxicol 1988;83:

-9.

Srivastava SP, Chen NO, Hotlzman JL. The in vitro NADPH

dependant inhibition by CCl4 of the ATP dependant Calcium

uptake of hepatic microsomes from male rats. Studies on the

mechanism o inactivation of the hepatic microsomal calcium pump

by CCl3. radical. J Biol Chem 1990;265:8392-9.

Saraswat B, Visen PK, Patnaik GK, Anticholestatic effect of

picroliv, active hepatoprotective principle of Picrorhiza kurrooa,

against carbon tetrachloride induced cholestasis. India J Exp Biol

;31:316-8.

DOI: https://doi.org/10.22377/ijgp.v3i2.68

Refbacks

There are currently no refbacks.

Username
Password
Remember me