Investigation of lipid profile and occular oxidative stress of Chloroxylon swietenia on Streptozotocin‑nicotinamide‑induced diabetic rats

Aravind Patchimatla, Sainath Reddy Kankanala, Sharavanabhava Sheshagiri Bandaru, Umasankar Kulindaivelu, Venkateshwar Rao Jupally, Venkateshwarlu Eggadi


Background: Diabetes mellitus is a chronic metabolic disorder, characterised by hyperglycaemia resulting from defects in insulin
secretion, insulin action or both. Aim: This investigation was designed to study the antidiabetic effect of Ethanolic extract of Chloroxylon swietenia (EECS) in Streptozotocin‑Nicotinamide‑induced  type‑II diabetes in rats. Materials and Methods: The extract at doses
of 250 and 500 mg/kg was given to the overnight‑fasted Wistar albino rats for 14 days and the antidiabetic, lipid profile and ocular oxidative stress in Streptozotocin‑Nicotinamide‑induced diabetic rats were evaluated. The parameters studied were blood glucose, lipid profile [total cholesterol (TC), triglyceride (TG), high‑density lipoprotein (HDL), low‑density lipoprotein (LDL) and very low‑density lipoprotein (VLDL)], serum enzymes such as serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), antioxidant enzymes like catalase (CAT), thiobarbituric acid reactive substances (TBARS), glutathione (GSH), complete blood picture (RBC, haemoglobin, WBC), insulin and liver glycogen levels. The result of test drug was compared with diabetic control. Glibenclamide (10 mg/kg) was selected as standard hypoglycaemic drug. Statistical Analysis: Results were expressed as Mean ± SD. Dunnet’s and one‑way ANOVA test was used to compare the mean values of test groups and diabetic control. Results: Administration of EECS prior to glucose overload resulted significant attenuation in blood sugar level at 60 and 120 min in comparison to glucose control
group. The antidiabetic activity of EECS showed significant (P < 0.001) reduction in blood glucose level at 250 mg/kg and 500 mg/kg dose levels at 14th day. EECS with (250 and 500 mg/kg) also decreased in serum SGOT, SGPT, TG, TC, VLDL‑C, LDL‑C, WBC and TBARS, in diabetic‑induced rats. In addition EECS at (250 and 500 mg/kg) increased liver glycogen, insulin, complete blood picture RBC, haemoglobin, ocular oxidative enzymes CAT, GSH and body weight when compared with diabetic control. Conclusion: The result obtained from the present study revealed the potential anti‑diabetic activity of Ethanolic extract of Chloroxylon swietenia.
Key words: Blood glucose, blood profile, Chloroxylon swietenia, diabetes mellitus, lipid profile, liver glycogen, ocular oxidative stress,
serum insulin

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Kaleem M, Medha P, Ahmed QU, Asif M, Bano B. Beneficial effects

of Annona squamosa extract in streptozotocin‑induced diabetic

rats. Singapore Med J 2008;49:800‑4.

Khan V, Najmi AK, Akhtar M, Aqil M, Mujeeb M, Pillai KK.

A pharmacological appraisal of medicinal plants with antidiabetic

potential. J Pharm Bioallied Sci 2012;4:27‑42.

Goycheva P, Gadjeva V, Popov B. Oxidative stress and its

complications in diabetes mellitus. Trakia J Sci 2006;4:1‑8.

Sandeep S, Mohan V. Changing trends epidemiology of diabetes.

Med Update 2008;18:133‑41.

Kiran SR, Devi PS, Reddy JK. Evaluation of in vitro antimicrobial

activity of leaf and stem essential oils of Chloroxylon swietenia DC.

World J Microbiol Biotechnol 2008;24:1909‑14.

Vinatha N, Estari M. An ethnobotanical study of plants used for

the treatment of diabetes in the Warangal district, Andhra Pradesh,

India. Biolife 2013;1:24‑8.

Ranjith KH, Surendra KP, Karthik SP, Rajendra J. Screening of

Chloroxylon swietenia DC root for antibacterial and anthelmintic

activities. Pharmacol Online 2011;1:544‑52.

Palani S, Raja S, Kumar SB. Hepatoprotective and antioxidant potential

of Chloroxylon swietenia (Rutaceae) on acetaminophen induced toxicity

in male albino rats. Int J Pharm Tech Res 2010;2:162‑70.

Ravi Kiran S, Bhavani K, Sita Devi P, Rajeswara Rao BR, Janardhan

Reddy K. Composition and larvicidal activity of leaves and stem

essential oils of Chloroxylon swietenia DC against Aedes aegypti and

Anopheles stephensi. Bioresour Technol 2006;97:2481‑4.

Kumar K, Ganesh M, Baskar S, Srinivasan K, Kanagasabai R,

Sambathkumar R, et al. Evaluation of anti‑inflammatory activity

and toxicity studies of Chloroxylon sweitenia in Rats. Anc Sci Life


Senthilraja A, Ramkumar R. Analgesic activity of Chloroxylon

swietenia. Agron 2003;40:32‑4.

Sharma AK, Chandan BK, Anand KK, Pushpangadan P.

Antifertility activity of Chloroxylon swietenia DC in rats. Zoolog

Orient 1989;4:50‑5.

Bonner‑Weir S. Morphological evidence of pancreatic polarity of

beta‑cell within islets of Langerhans. Diabetes 1988;37:616‑21.

Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark

extracts of Garuga pinnata Roxb. in streptozotocin‑ nicotinamide

induced type‑II diabetes mellitus. J Ethnopharmacol


Trinder P. Determination of glucose in blood using glucose oxidase

with alternate oxygen receptor. Ann Clin Chem 1969;6:24‑7.

Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic

determination of total serum cholesterol. Clin Chem 1974;20:470‑5.

Werner M, Gabrielson DG, Eastman J. Ultramicro determination

of serum triglycerides by bioluminescent assay. Clin Chem


Friedwald WT, Levy RI, Fredrickson DS. Estimation of

the concentration of low density lipoprotein cholesterol in

plasma, without use of preparative ultracentrifuge. Clin Chem


Reitman S, Frankel S. A colorimetric method for the determination

of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin Pathol 1957;28:56‑63.

Morales MA, Jabbay AJ, Tenenzi HP. Mutation affecting

accumulation of glycogen. Neurospora News Lett 1973;20:24‑5.

Chigozie IJ, Chidinma IC. Positive moderation of the hematology,

plasma biochemistry and ocular indices of oxidative stress in

alloxan‑induced diabetic rats, by an aqueous extract of the leaves

of Sansevieria liberica Gerome and Labroy. Asian Pac J Trop Med


Ikewuchi CC, Onyeike EN, Uwakwe AA, Ikewuchi CC.

Effect of aqueous extract of the leaves of Acalypha wilkesiana

‘Godseffana’ Muell Arg (Euphorbiaceae) on the hematology, plasma

biochemistry and ocular indices of oxidative stress in alloxan

induced diabetic rats. J Ethnopharmacol 2011;137:1415‑24.

Ikewuchi JC. Moderation of hematological and plasma biochemical

indices of sub chronic salt‑loaded rats, by an aqueous extract

of the leaves of Acalypha wilkesiana ‘Godseffiana’ Muell

Arg (Euphorbiaceae). Asian Pac J Trop Med 2013;6:37‑42.

Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121‑6.

Beutler E, Duron O, Kelly BM. Improved method for the

determination of blood glutathione. J Lab Clin Med 1963;61:882‑8.

Satoh K. Serum lipid peroxide in cerebrovascular disorders

determined by a new colorimetric method. Clin Chim Acta


Raghvan B, Krishnakumari S. Hypoglycemic and hypolipidemic

activities of Terminalia arjuna stem bark in alloxan induced diabetic

rats. J Nat Remedies 2006;6:124‑30.

Pullaiah T, Naidu KC. Antidiabetic plants in India and herbal based

antibacterial research. New Delhi: Regency Publication; 2003.314‑5.

Venkateshwarlu E, Sharvanabhava BS, Arvind P, Rakeshkumar

Reddy P, Dileep P, Mahathi K. Evaluation of anti‑diabetic and

hypolipidemic activity of Pseudarthria viscida (whole plant) in

streptozotocin‑nicotinamide induced Type II diabetic rats. Glob J

Pharmacol 2013;7:192‑7.

Grover JK, Vats V, Rathi SS. Anti‑hyperglycemic effects of Eugenia

jambolana and Tinospora cardiofolia in experimental diabetes and

their effects on key enzymes involved in carbohydrate metabolism.

J Ethnopharmacol 2000;73:461‑70.

Shukla R, Anand K, Prabhu KM, Murthy PS. Hypolipidemic effect

of water extracts of Ficus bengalensis in alloxoaninduced diabetes

mellitusin rabbits. Indian J Clin Biochem 1995;10:119‑21.

Ghosh S, Suryawanshi SA. Effect of Vinca rosea extracts in

treatment of alloxan diabetes in male albino rats. Indian J Exp Biol


Venkateshwarlu E, Dileep P, Rakeshkumar Reddy P, Sandhya P.

Evaluation of antidiabetic acivity of Carica papaya seeds on

streptozotocin induced Type‑II diabetic rats. J Adv Sci Res


Vijayakumar M, Govindrajan R, Rao GM, Rao ChV, Shirwaikar A,

Mehrota S, et al. Action of Hygrophila auriculata against

streptozotocin‑induced oxidative stress. J Ethnophamacol


Arias IM, Jakoby WB. Determination of tissue glutathione.

Glutathione: Metabolism and functions. New York: Raven Press;

p. 115‑38.

Loven D, Schedl H, Wilson H, Daabees TT, Stegink LD, Diekus M,

et al. Effect of insulin and oral glutathione on glutathione levels

and superoxide dismutase activities in organs of rats with

streptozotocin‑induced diabetes. Diabetes 1986;35:503‑7.



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