Ultrasound‑assisted extraction and fast chromolithic method development, validation and system suitability analysis for 6, 8, 10‑gingerols and shogaol in rhizome of Zingiber officinale by liquid chromatography‑diode array detection

Pankaj Pandotra, Rajni Sharma, Prabhu Datt, Manoj Kushwaha, Ajai Prakash Gupta, Suphla Gupta

Abstract


Background: Ginger, the rhizome of Zingiber officinale Roscoe (family Zingeberace) has been used as a spice throughout the world since times immemorial. Ginger has been valued as a medicinal herb in several countries and has been reported to possess carminative, anti‑emetic, anti‑nauseate and anti‑inflammatory properties. Aim: Chromolith reverse phase liquid chromatographic method for simultaneous determination, quantification and validation was developed for 6, 8, 10‑gingerols and shogaol in the dry rhizome of Zingiber officinale. Materials and Methods: The method was in accordance to the International Conference on Harmonisation. It is highly specific, exhibited good linearity’s (r2 > 0.9998) with high precision and achieved good accuracies between 97.81 and 100.84% of quantitative results. For the developed chromolith LC method, system suitability parameters like K prime, selectivity, resolution, USP resolution, asymmetry, USP tailing, symmetry factor, USP plate count and peak purity were also calculated.
Results and Conclusions: The developed chromolith method was more sensitive, 4‑times faster, generated very good number of
theoretical plates (4041 to 22364) and gave better peak resolutions as compared to the earlier methods developed on normal LC columns.
Key words: Chromolith, RP‑LC‑PDA, rhizome, ultra‑sound assisted, Z. officinale

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DOI: http://dx.doi.org/10.22377/ijgp.v7i3.321

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