Pigeon pea seed husks as potent natural resource of anti‑oxidant and anti‑hyperglycaemic activity

Ashok Kumar Tiwari, Bacha Abhinay, Katragadda Suresh Babu, Domati Anand Kumar, Amtul Zehra, Kuncha Madhusudana

Abstract


Background: Pigeon pea (Cajanus cajan L. Millspaugh) is an important food legume. Its seed, root and leaves have been explored for various medicinal properties. Pigeon pea seed husks constitute the major by‑product of milling industries and are mainly used as cattle feed.

Aim: To explore anti‑oxidant and anti‑hyperglycaemic potential in pigeon pea seed husks. Materials and Methods: Methanolic
extracts of pigeon pea seed husks were evaluated for various phytochemical constituents, free radicals scavenging and anti‑oxidant potentials in vitro. Crude methanol extract was further evaluated for its anti‑hyperglycaemic activity in starch‑induced postprandial
hyperglycaemic rats. Results: Methanolic extracts of seed husks were found to be the rich source of polyphenols and protein and
therefore possess potent free radicals scavenging, anti‑oxidant activities in vitro. Furthermore, crude methanol extract of pigeon pea seed husks significantly (P < 0.05) mitigated starch‑induced postprandial glycaemic excursions and reduced glycaemic load in rats similar to the standard drug acarbose. Conclusion: Observations made in this study indicate that husks of pigeon pea seeds may serve as potential natural resource for the development of anti‑oxidant rich anti‑hyperglycaemic functional food and/or nutraceuticals.
Key words: Anti‑hyperglycaemic activity, anti‑oxidant activity, Cajanus cajan, pigeon pea seed husks


Full Text:

PDF

References


Saxena KB, Vijaya KR, Sultana R. Quality nutrition through pigeon

pea: A review. Health 2010;2:1335‑44.

Faris DG, Singh U. Pigeon pea: Nutrition and products. In:

Nene YL, Hall SD, Sheila VK, editors. The Pigeon Pea, Wallingford:

CAB International; 1990. p. 401‑34.

FAO, 2008. Available from: http://www.faostat.fao.org/. [Last

accessed on 2013 Jun 12].

Duke JA. Hand book of legumes of world economic importance.

New York: Plenum Press; 1981.

Amalraj T, Ignacimuthu S. Evaluation of the hypoglycemic effect

of Cajanus cajan (seeds) in mice. Indian J Exp Biol 1988;36:1032‑3.

Grover JK, Yadav S, Vats VJ. Medicinal plants of India with

anti‑diabetic potential. J Ethnopharmacol 2002;81:81‑100.

Edbordo CR. Medicinal plant of the Philippines. Quezon City:

Katha Publishing Company Inc; 1978.

Chen DH, Li HY, Lin H. Studies on chemical constituents in pigeon

pea leaves. Chin Tradit Herb Drugs 1985;16:134‑6.

Li ZH, Zhou CH, Gu Y, Zhang JY. The present status of study and

utilization of pigeon pea in China and its prospects. Forest Res

;14:674‑81.

Wu N, Fu K, Jie FY, Gang ZY, Chang FR, Chen YH, et al.

Antioxidant activities of extracts and main components of Pigeon

pea [Cajanus cajan (L.) Millsp] leaves. Molecules 2009;1:1032‑43.

Liu W, Kong Y, Zu Y, Fu Y, Luo Y, Zhang Y, et al. Determination and

quantification of active phenolic compounds in pigeon pea leaves

and its medicinal product using liquid chromatography–tandem

mass spectrometry. J Chromatogr A 2010;1217:4723‑31.

Ahalya N, Kanamadi RD, Ramachandra TV. Cr (VI) and Fe (III)

removal using Cajanus cajan husk. J Environ Biol 2007;28:765‑9.

Tiwari BK, Brennan CS, Jaganmohan R, Surabi A, Alagusundaram K.

Utilisation of pigeon pea (Cajanus cajan L.) by products in biscuit

manufacture. Food Sci Technol Int 2011;44:1533‑7.

Kanatt SR, Arjun K, Sharma A. Antioxidant and antimicrobial

activity of legume hulls. Food Res Int 2011;44:3182‑7.

Tiwari AK, Reddy SK, Janani R, Anand DK, Zehra A, Agawane SB,

et al. Influence of antioxidant rich vegetables fresh juice on

starch induced postprandial hyperglycemia in Rats. Food Funct

;2:521‑8.

Tiwari AK, Swapna M, Ayesha SB, Zehra A, Agawane SB,

Madhusudana K. Identification of proglycemic and

antihyperglycemic activity in antioxidant rich fraction of some

common food grains. Int Food Res J 2011;18:915‑23.

Tiwari AK, Praveen MK, Anand DK, Agawane SB, Madhusudana K,

Zehra A. Ayurvedic dietary formulations and postprandial

glycemia in rats. Int Food Res J 2012;19:765‑73.

Tiwari AK, Anusha I, Sumangali M, Anand DK, Madhusudana K,

Agawane SB. Preventive and therapeutic efficacies of Benincasa

hispida and Sechium edule fruit’s juice on sweet‑beverages induced

impaired glucose tolerance and oxidative stress. Pharmacologia

;4:197‑207.

Tiwari AK, Manasa K, Anand DK, Zehra A. Raw horse gram seeds

possess more in vitro antihyperglycemic activities and antioxidant

properties than their sprouts. Nutrafoods 2013;12:47‑54.

Yao Y, Sang W, Zhou M, Ren G. Antioxidant and ά‑glucosidase

inhibitory activity of colored grains in China. J Agric Food Chem

;58:770‑4.

Giusti MM, Luis E, Saona R, Wrolstad RE. Molar absorptivity and

color characteristics of acylated and non‑acylated pelargonidin‑based

anthocyanins. J Agric Food Chem 1999;47:4631‑7.

Bradford MM. A rapid and sensitive method for the quantification

of microgram quantities of protein utilizing the principle of protein

dye binding. Anal Biochem 1976;72:248‑54.

Walker RB, Everette JD. Comparative reactions rates of various

antioxidants with ABTS radical cation. J Agric Food Chem

;57:1156‑61.

Asgary S, Naderi GH, Sarrafzadegan N, Ghassemi N, Boshtam M,

Rafie M, et al. Anti‑oxidant effect of flavonoids on haemoglobin

glycosylation. Pharma Acta Helvet 1991;73:223‑6.

Zimmet PZ, Alberti KG. Introduction: Globalization and the

non‑communicable disease epidemic. Obesity (Silver Spring)

;14:1‑3.

Gujral UP, Pradeepa R, Weber MB, Narayan KM, Mohan V. Type 2

diabetes in South Asians: Similarities and differences with white

Caucasian and other populations. Ann NY Acad Sci 2013;1281:51‑63.

Sivasankaran S. The cardio‑protective diet. Indian J Med Res

;132:608‑16.

Burden ML, Samanta A, Spalding D, Burden AC. A comparison of

the glycemic and insulinaemic effects of an Asian and a European

meal. Pract Diab Int 1994;11:208‑11.

Mohan V, Radhika G, Sathya RM, Tamil SR, Ganesan A, Sudha V.

Dietary carbohydrates, glycemic load, food groups and newly

detected type 2 diabetes among urban Asian Indian population

in Chennai, India (Chennai Urban Rural Epidemiology Study 59).

Br J Nutr 2009;102:1498‑506.

Carroll MF, Gutierrez A, Castro M, Tsewang D, Schade DS.

Targeting postprandial hyperglycemia: A comparative study of

insulinotropic agents in type 2 diabetes. J Clin Endocrinol Metab

;88:5248‑54.

O’Keefe JH, Gheewala NM, O’Keefe JO. Dietary strategies for

improving post‑prandial Glucose, Lipids, Inflammation, and

cardiovascular health. J Am Coll Cardiol 2008;51:249‑55.

Ceriello A. The post‑prandial state and cardiovascular disease:

Relevance to diabetes mellitus. Diabetes Metab Res Rev

;16:125‑32.




DOI: http://dx.doi.org/10.22377/ijgp.v7i3.331

Refbacks

  • There are currently no refbacks.