Mast cell stabilising activity of various subfractions of leaves of Vitex Negundo

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DOI: https://doi.org/10.22377/ijgp.v5i4.212

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Jignesh I. Patel
Shrikalp S. Deshpande

Abstract

To evaluate anti-asthmatic, anti-inflammatory, and anti-allergic activity of various subfractions of ethyl acetate fraction of leaves of Vitex negundo. Ethyl acetate fraction (EAF) of leaves of V. negundo was further subfractionated into NaOH fraction, acetone fraction, and chloroform fraction to separate chemical constituents present in EAF using thin layer chromatography technique and different solvent systems. The stabilisation potential of the various subfractions of EAF of leaves of V. negundo against mast cell degranulation
was studied on isolated mesentery of rat by exposure to compound 48/80. Dexamethasone (5 mg/kg) was used as a reference standard. AF of EAF of leaves of V. negundo (500 μg/ml) showed significant protection of rat mesenteric mast cells from disruption caused by compound 48/80. This study concluded that AF of EAF of leaves of V. negundo shows mast cell stabilising activity. It may be useful
as anti-asthmatic, anti-inflammatory, and anti-allergic agent.
Key words: Asthma, compound 48/80, mast cell, Vitex negundo

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Vol. 5 No. 4 (2011): Oct-Dec

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