Antidiabetic activity and modulation of antioxidant status by fractions of Argemone mexicana in alloxan induced diabetic rats

Praveen Singh Nayak, Durga Madhab Kar, Shweta P. Nayak


Background: Argemone mexicana L. (Papaveraceae) commonly known as prickly poppy is an indigenous herb used as a medicinal
plant in several countries. Aim: The investigation was carried out to study the effects of chloroform and aqueous soluble fractions from hydroalcoholic extract of Argemone mexicana in normoglycemic and alloxan induced diabetic rats. It was also intended to establish correlation between the marker antioxidant enzymes and diabetes. Materials and Methods: Hyperglycemia was induced in rats by alloxan monohydrate (150mg/kg body weight i.p.). After alloxan induction diabetic rats received chloroform and aqueous fractions orally at 150 mg/kg body weight daily for 21days. The parameters studied were blood glucose, creatinine and urea, serum lipid profile,
serum enzymes [serum glutamate pyruvate transaminases and serum glutamate oxaloacetate transaminases, lipid peroxidation, antioxidant enzymes (catalase (CAT), superoxide dismutase and reduced glutathione. The results of test drug were compared with standard hypoglycaemic drug-glibenclamide (5 mg/kg). Statistical analysis: All data were expressed as means ± SEM. Dunnet’s t-test and one-way ANOVA test was used to compare the mean values of test groups and control. Results: . Experimental findings showed that the chloroform and aqueous soluble fractions significantly (P < 0.01)normalized blood glucose levels, serum biochemical parameters; decreased LPO and recovered glutathione‑S‑tranferase (GST) and CAT as compared with those of alloxan controls..

Conclusion: From this study it may be concluded that the potential anti‑diabetic action of chloroform and aqueous fractions of A. Mexicana is plausibly due to its modulation of endogenous antioxidant status
Key words: Anti‑diabetic activity, Argemone mexicana, alloxan monohydrate

Full Text:



Misra MK. Need for conservation of indigenous medicinal

knowledge and the herbs. J Hum Ecol 1999;10:403‑6.

Murugesh K, Yeligar V, Dash DK, Sengupta P, Maiti BC, Maity TK.

Antidiabetic, antioxidant and antihyperlipidemic status of

Heliotropium zeylanicum extract on streptozotocin‑induced

diabetes in rats. Biol Pharm Bull 2006;29:2202‑5.

Grover JK, Yadav S, Vats V. Medicinal plants of India with

anti‑diabetic potential. J Ethnopharmacol 2002;81:81‑100.

Alberto JN. Antioxidant therapy myth or reality. J Braz Chem Soc


Marx JL. Oxygen free radicals linked to many diseases. Science


Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal

Plants (Including the Supplement). New Delhi: Council of

Scientific and Industrial Research; 1986. p. 23.

WHO. The promotion and development of traditional medicine.

Technical Report Series Published by world health organization,

Geneva; 978. p. 622.

Shane MC, Whorter L. Biological complementary therapies a focus

on botanical products in diabetes. Diabetes Spectr 2001;14:199‑208.

Ecobichon DJ. Acute Toxicity Studies. The Basis of Toxicology

Testing. 3rded. New York: CRC Press; 1997. p. 43‑86.

Nagappa AN, Thakurdesai PA, Venkat Rao N, Singh J. Antidiabetic

activity of Terminalia catappa Linn fruits. J Ethnopharmacol


Aslan M, Deliorman Orhan D, Orhan N, Sezik E, Yesilada E. In vivo

antidiabetic and antioxidant potential of Helichrysum plicatum

ssp. plicatum capitulums in streptozotocin‑induced‑diabetic rats.

J Ethnopharmacol 2007;109:54‑9.

Aslan M, Orhan DD, Orhan N, Sezik E, Yeşilada E. A study of

antidiabetic and antioxidant effects of Helichrysum graveolens

capitulums in streptozotocin‑induced diabetic rats. J Med Food


Anreddy RN, Porika M, Yellu NR, Devarakonda RK. Hypoglycemic

and hypolipidemic activities of Trianthemaportulacastrum Linn.

Plant in normal and alloxan induced diabetic rats. IntJPharmacol


Sood R. Clinical chemistry. In: Medical Laboratory Technology,

Methods and Interpretations. 5th ed. India: Jaypee Brothers’

Medical Publishers; 1999. p. 173‑404.

Reitman S, Frankel S. A colorimetric method for the determination

of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin Pathol 1957;28:56‑63.

Owen Ja, Iggo B, Scandrett FJ, Stewart CP. The determination of

creatinine in plasma or serum, and in urine; a critical examination.

Biochem J 1954;58:426‑37.

Sedlak J, Lindsay RH. Estimation of total, protein‑bound, and

nonprotein sulfhydryl groups in tissue with Ellman’s reagent.

Anal Biochem 1968;25:192‑205.

Ellman Gl. Tissue sulfhydryl groups. Arch Biochem Biophys


Pinto RE, Bartley W. The effect of age and sex on glutathione

reductase and glutathione peroxidase activities and on aerobic

glutathione oxidation in rat liver homogenates. Biochem J


Heikkila RE, Cabbat F. A sensitive assay for superoxide dismutase

based on the autoxidation of 6‑hydroxydopamine. Anal Biochem


Aebi H. Catalase in‑vitro.MethodsEnzymol 1984;105:121‑6.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein

measurement with the Folin phenol reagent. J Biol Chem


Ghosh D, Bera TK, Chatterjee K, Ali KM, De D. Antidiabetic and

antioxidant effects of aqueous extract of seed of Psoralea corylifolia

and seed of Trigonella foenumgraecum L. (Methi) in separate and

composite in manner in streptozotocin induced diabetic mice. Int

J Pharm Res Devel 2009;7:1‑10.

Nayak P, Kar DM, Maharana L. Antidiabetic activity of aerial parts

of Argemone alloxan induced hyperglycaemic rats.

Pharmacol Online 2011;1:889‑903.

Shankar MB, Parikh JR, Geetha M, Mehta RS, Saluja AK.

Anti‑diabetic activity of novel androstane derivatives from

Syzygium cuminii Linn. J Nat Remedies 2007;7:214‑9.

Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative

effect of Diasulin, a polyherbal formulation in alloxan induced

hyperglycemic rats. BMC Complement Altern Med 2005;5:14.

Ghosh S, Suryawanshi SA. Effect of Vinca rosea extracts in

treatment of alloxan diabetes in male albino rats. Indian J Exp Biol


Sharma SR, Dwivedi SK, Swarup D. Hypoglycaemic,

antihyperglycaemic and hypolipidemic activities of Caesalpinia

bonducella seeds in rats. J Ethnopharmacol 1997;58:39‑44.

Arvind K, Pradeepa R, Deepa R, Mohan V. Diabetes and coronary

artery disease. Indian J Med Res 2002;116:163‑76.

Mironova MA, Klein RL, Virella GT, Lopes‑Virella MF.

Anti‑modified LDL antibodies, LDL‑containing immune

complexes, and susceptibility of LDL to in vitro oxidation in

patients with type 2 diabetes. Diabetes 2000;49:1033‑41.

Scott M, Grundy MD.Diabetis cardiovascular disease. Circulation


Arias IM, Jakoby WB. Detrmination of Tissue Glutathione.

Glutathione: Metabolism and Functions. New York: Raven Press;

p. 11.

Loven D, Schedl H, Wilson H, Daabees TT, Stegink LD, Diekus M,

et al. Effect of insulin and oral glutathione on glutathione levels

and superoxide dismutase activities in organs of rats with

streptozocin‑induced diabetes. Diabetes 1986;35:503‑7.

Arulselvan P, Subramanian SP. Beneficial effects of Murraya koenigii

leaves on antioxidant defense system and ultra structural changes

of pancreatic beta‑cells in experimental diabetein rats. Chem Biol

Interact 2007;165:155‑64.

Manonmani G, Bhavapriya V, Kalpana S, Govindasamy S,

Apparanantham T. Antioxidant activity of Cassia fistula (Linn.)

flowers in alloxan induced diabetic rats. J Ethnopharmacol


Halliwell B, Gutteridge JM. Lipid peroxidation, oxygen radicals,

cell damage and antioxidant therapy. Lancet 1984;1:1396‑7.

Wohaieb SA, Godin DV. Alterations in free radical tissue‑defense

mechanisms in streptozocin‑induced diabetes in rat. Effects of

insulin treatment. Diabetes 1987;36:1014‑18.

Yoshikawa T, Naito Y, Kishi A, Tomii T, Kaneko T, Iinuma S, et al.

Role of active oxygen, lipid peroxidation, and antioxidants in the

pathogenesis of gastric mucosal injury induced by indomethacin

in rats. Gut 1993;34:732‑7.



  • There are currently no refbacks.